Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers

Amos Mugweru; Bingquan Wang; James Rusling

doi:10.1021/ac049375j

Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers

Amos Mugweru, Bingquan Wang, James Rusling

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Films containing [Os(bpy)₂(PVP)₁₀Cl]^- and [Ru(bpy)₂(PVP)₁₀Cl]⁺ metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive Os³⁺/Os²⁺ and Ru ³⁺/Ru²⁺ centers, with formal potentials of 0.34 and 0.76 Vvs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleobases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.

Original language	English (US)
Pages (from-to)	5557-5563
Number of pages	7
Journal	Analytical Chemistry
Volume	76
Issue number	18
DOIs	https://doi.org/10.1021/ac049375j
State	Published - Sep 15 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac049375j

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title = "Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers",

abstract = "Films containing [Os(bpy)2(PVP)10Cl]- and [Ru(bpy)2(PVP)10Cl]+ metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive Os3+/Os2+ and Ru 3+/Ru2+ centers, with formal potentials of 0.34 and 0.76 Vvs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleobases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.",

author = "Amos Mugweru and Bingquan Wang and James Rusling",

year = "2004",

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doi = "10.1021/ac049375j",

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AU - Mugweru, Amos

AU - Wang, Bingquan

AU - Rusling, James

PY - 2004/9/15

Y1 - 2004/9/15

N2 - Films containing [Os(bpy)2(PVP)10Cl]- and [Ru(bpy)2(PVP)10Cl]+ metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive Os3+/Os2+ and Ru 3+/Ru2+ centers, with formal potentials of 0.34 and 0.76 Vvs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleobases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.

AB - Films containing [Os(bpy)2(PVP)10Cl]- and [Ru(bpy)2(PVP)10Cl]+ metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive Os3+/Os2+ and Ru 3+/Ru2+ centers, with formal potentials of 0.34 and 0.76 Vvs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleobases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.

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Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers

Abstract

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