Voltammetric sensor for oxidized DNA using ultrathin films of osmium and ruthenium metallopolymers

Amos Mugweru, Bingquan Wang, James Rusling

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Films containing [Os(bpy)2(PVP)10Cl]- and [Ru(bpy)2(PVP)10Cl]+ metallopolymers were assembled layer by layer on pyrolytic graphite electrodes to make sensors that selectively detect oxidized DNA. These films showed reversible, independent electrochemistry for electroactive Os3+/Os2+ and Ru 3+/Ru2+ centers, with formal potentials of 0.34 and 0.76 Vvs SCE, respectively. The combination of ruthenium and osmium metallopolymers in the films provided a catalytic Os square wave voltammetry (SWV) peak that is mainly selective for 8-oxoguanine and the detection of other oxidized nucleobases from the Ru peak. The method is applicable to measurements on DNA in solution or DNA incorporated into films. Using the Os SWV peak, 1 oxidized nucleobase in 6000 was detected. The sensor is simple and inexpensive, and the approach may be useful for the detection of oxidized DNA as a clinical biomarker for oxidative stress.

Original languageEnglish (US)
Pages (from-to)5557-5563
Number of pages7
JournalAnalytical Chemistry
Volume76
Issue number18
DOIs
StatePublished - Sep 15 2004

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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