Vitamin D deficiency in a multiethnic healthy control cohort and altered immune response in vitamin D deficient European-American healthy controls

Lauren L. Ritterhouse, Rufei Lu, Hemangi B. Shah, Julie M. Robertson, Dustin A. Fife, Holden T. Maecker, Hongwu Du, Charles G. Fathman, Eliza F. Chakravarty, R. Hal Scofield, Diane L. Kamen, Joel M. Guthridge, Judith A. James

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Objective: In recent years, vitamin D has been shown to possess a wide range of immunomodulatory effects. Although there is extensive amount of research on vitamin D, we lack a comprehensive understanding of the prevalence of vitamin D deficiency or the mechanism by which vitamin D regulates the human immune system. This study examined the prevalence and correlates of vitamin D deficiency and the relationship between vitamin D and the immune system in healthy individuals. Methods: Healthy individuals (n = 774) comprised of European-Americans (EA, n = 470), African Americans (AA, n = 125), and Native Americans (NA, n = 179) were screened for 25-hydroxyvitamin D [25(OH)D] levels by ELISA. To identify the most noticeable effects of vitamin D on the immune system, 20 EA individuals with severely deficient (<11.3 ng/mL) and sufficient (>24.8 ng/mL) vitamin D levels were matched and selected for further analysis. Serum cytokine level measurement, immune cell phenotyping, and phosphoflow cytometry were performed. Results: Vitamin D sufficiency was observed in 37.5% of the study cohort. By multivariate analysis, AA, NA, and females with a high body mass index (BMI, >30) demonstrate higher rates of vitamin D deficiency (p,0.05). Individuals with vitamin D deficiency had significantly higher levels of serum GM-CSF (p = 0.04), decreased circulating activated CD4+ (p = 0.04) and CD8+ T (p = 0.04) cell frequencies than individuals with sufficient vitamin D levels. Conclusion: A large portion of healthy individuals have vitamin D deficiency. These individuals have altered T and B cell responses, indicating that the absence of sufficient vitamin D levels could result in undesirable cellular and molecular alterations ultimately contributing to immune dysregulation.

Original languageEnglish (US)
Article numbere94500
JournalPloS one
Volume9
Issue number4
DOIs
StatePublished - Apr 11 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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