Two-dimensional separation techniques using supercritical fluid chromatography

Alexander S. Kaplitz, Mahmoud Elhusseiny Mostafa, Samantha A. Calvez, James L. Edwards, James P. Grinias

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations


High-resolution separation systems are essential for the analysis of complex mixtures in a wide variety of application areas. To increase resolution, multidimensional chromatographic techniques have been one key solution. Supercritical fluid chromatography provides a unique opportunity in these multidimensional separations based on its potential for high solvent compatibility, rapid duty cycles, and orthogonality to other separation modes. This review focuses on two-dimensional chromatography methods from the past decade that use supercritical fluid chromatography because of these advantages. Valving schemes and modulation strategies used to interface supercritical fluid chromatography with other liquid chromatography and gas chromatography techniques are described. Particular applications of multidimensional separations using supercritical fluid chromatography for the analysis of oils and chiral separations of pharmaceutical compounds are highlighted. Limitations of and a potential trajectory for supercritical fluid chromatography in this field are also discussed.

Original languageEnglish (US)
Pages (from-to)426-437
Number of pages12
JournalJournal of Separation Science
Issue number1
StatePublished - Jan 2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation


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