Transforming growth factor-β1 (TGF-β1) regulates ATDC5 chondrogenic differentiation and fibronectin isoform expression

Fei Han, Christopher S. Adams, Zhuliang Tao, Charlene J. Williams, Raihana Zaka, Rocky S. Tuan, Pamela A. Norton, Noreen J. Hickok

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31 Scopus citations


Regulated splicing of fibronectin (FN) occurs during the mesenchymal to chondrocyte transition and ultimately results in the relative enrichment of an extra domain B (EDB) exon-containing FN isoform with the suggestion that FN isoforms may play a functional role in chondrogenesis. Promotion of chondrogenesis can also be achieved by treatment with transforming growth factor-β (TGF-β), which also regulates FN isoform expression. We have examined the effects of TGF-β treatment on the assumption of the chondrogenic phenotype in the teratoma-derived cell line ATDC5 and tested whether these effects on chondrogenesis are paralleled by appropriate changes in FN isoform expression. ATDC5 cells were maintained in a pre-chondrogenic state and, in this state, treated with 10 ng/ml TGF-β. The cells started to elaborate a matrix rich insulfated proteoglycans, such that within the first 12 days of culture, TGF-β1 treatment appeared to slightly accelerate early acquisition of an Alcian blue-stained matrix, and caused a dose- and time-dependent decrease in collagen type I expression; changes in collagen type II expression were variable. At later times, cells treated with TGF-β became indistinguishable from those of the controls. Interestingly, TGF-β treatment caused a significant dose- and time-dependent decrease in the proportion of FN containing the extra domain A (EDA) and the EDB exons. These data suggest that TGF-β induces the early stages of chondrogenic maturation in this pre-chondrogenic line and that TGF-β treatment increases expression of FN isoforms that lack the EDA and EDB exons.

Original languageEnglish (US)
Pages (from-to)750-762
Number of pages13
JournalJournal of Cellular Biochemistry
Issue number4
StatePublished - Jul 1 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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