Tissue engineering of heart valve leaflet by self-assembly of tissue spheroids biofabricated from human fat tissue derived stem cells

V. Mironov, V. Kasyanov, A. Nagy-Mehesz, R. Moreno, Z. Hajdu, T. Trusk, I. Ozolanta, M. Murovska, Y. Wu, H. Yao, V. Beachley, X. Wen, A. Bradshaw, R. Visconti, R. Norris, R. Markwald

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Bioengineering of living heart valve substitute suitable for implantation into pediatric patients with heart valve diseases is an unsolved challenge in cardiovascular tissue engineering. In order to identify desirable material properties of such construct, the biomechanical, histological, histochemical and biochemical properties of fetal, perinatal and adult porcine heart valve leaflets have been systematically investigated. It have been shown that increasing in heart valve stiffness during ontogenesis correlates with accumulation of collagen type 1 and increasing level of collagen cross-linking. Tissue spheroids biofabricated from human fat tissue derived stem cells have been employed to engineer leaflet-like construct on compliant electrospun scaffold using self-assembly or tissue fusion approach. Incubation of tissue engineered heart valve leaflet construct with TGFβ as well as periostin transfection significantly increased stiffness of tissue engineered heart valve leaflet. These data demonstrated that the heart valve can be biofabricated by tissue fusion or self-assembly of closely placed tissue spheroids and that chemically and genetically induced accelerated tissue maturation and collagen deposition can enhance mechanical properties of tissue engineered leaflet. Further optimization of accelerated tissue maturation technologies could eventually lead to development of living autologous human heart valve tissue engineered construct with natural- like biomechanical properties suitable for pediatric cardiac patients.

Original languageEnglish (US)
Title of host publicationWorld Congress on Medical Physics and Biomedical Engineering
Subtitle of host publicationBiomaterials, Cellular and Tissue Engineering, Artificial Organs
Pages230-232
Number of pages3
Edition10
DOIs
StatePublished - Dec 1 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs - Munich, Germany
Duration: Sep 7 2009Sep 12 2009

Publication series

NameIFMBE Proceedings
Number10
Volume25
ISSN (Print)1680-0737

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs
CountryGermany
CityMunich
Period9/7/099/12/09

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All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering

Cite this

Mironov, V., Kasyanov, V., Nagy-Mehesz, A., Moreno, R., Hajdu, Z., Trusk, T., Ozolanta, I., Murovska, M., Wu, Y., Yao, H., Beachley, V., Wen, X., Bradshaw, A., Visconti, R., Norris, R., & Markwald, R. (2009). Tissue engineering of heart valve leaflet by self-assembly of tissue spheroids biofabricated from human fat tissue derived stem cells. In World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs (10 ed., pp. 230-232). (IFMBE Proceedings; Vol. 25, No. 10). https://doi.org/10.1007/978-3-642-03900-3-66