The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay

Carlos I. González, María J. Ruiz-Echevarría, Shobha Vasudevan, Michael F. Henry, Stuart W. Peltz

Research output: Contribution to journalArticle

121 Scopus citations

Abstract

The nonsense-mediated mRNA decay (NMD) pathway monitors premature translation termination and degrades aberrant mRNAs. In yeast, it has been proposed that a surveillance complex searches 3' of a nonsense codon for a downstream sequence element (DSE) associated with RNA-binding proteins. An interaction between the complex and the DSE-binding protein(s) triggers NMD. Here we describe the identification and characterization of the Hrp1/Nab4 protein as a DSE-binding factor that activates NMD. Mutations in HRP1 stabilize nonsense-containing transcripts without affecting the decay of wild-type mRNAs. Hrp1p binds specifically to a DSE-containing RNA and interacts with Upf1p, a component of the surveillance complex. A mutation in HRP1 that stabilizes nonsense-containing mRNAs abolishes its affinity for the DSE and fails to interact with Upf1p. We present a model describing how Hrp1p marks a transcript for rapid decay.

Original languageEnglish (US)
Pages (from-to)489-499
Number of pages11
JournalMolecular Cell
Volume5
Issue number3
DOIs
StatePublished - Jan 1 2000

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this