The use of optical emission spectroscopy for human 15N tracer studies

T. P. Stein; M. J. Leskiw; E. M. Liquori; H. B. Brooks; H. W. Wallace; W. S. Blakemore

doi:10.1016/0003-2697(75)90615-6

The use of optical emission spectroscopy for human ¹⁵N tracer studies

T. P. Stein, M. J. Leskiw, E. M. Liquori, H. B. Brooks, H. W. Wallace, W. S. Blakemore

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Optical emission spectroscopy is a convenient method for determing ¹⁵N specific activity in a variety of metabolites following the administration of an ¹⁵N-labeled amino acid to humans. The only disadvantage, compared to the more conventional isotope ratio mass spectrometer analytical system is that more tracer is needed to give accurate results. When an ¹⁵N-labeled amino acid is used as the ¹⁵N carrier, the amount of ¹⁵N is not above a tracer dose. A suitable procedure is described for performing such studies using [¹⁵N]glycine as a tracer. As an experimental example, [¹⁵N]glycine was infused into a subject at a rate of 35 mg of N/hr for 8 hr. After 3 hr a meal was consumed by the subject. The urinary urea-¹⁵N, ammonia-¹⁵N and amino acid-¹⁵N profiles were determined.

Original language	English (US)
Pages (from-to)	481-488
Number of pages	8
Journal	Analytical Biochemistry
Volume	66
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(75)90615-6
State	Published - Jun 1975
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(75)90615-6

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title = "The use of optical emission spectroscopy for human 15N tracer studies",

abstract = "Optical emission spectroscopy is a convenient method for determing 15N specific activity in a variety of metabolites following the administration of an 15N-labeled amino acid to humans. The only disadvantage, compared to the more conventional isotope ratio mass spectrometer analytical system is that more tracer is needed to give accurate results. When an 15N-labeled amino acid is used as the 15N carrier, the amount of 15N is not above a tracer dose. A suitable procedure is described for performing such studies using [15N]glycine as a tracer. As an experimental example, [15N]glycine was infused into a subject at a rate of 35 mg of N/hr for 8 hr. After 3 hr a meal was consumed by the subject. The urinary urea-15N, ammonia-15N and amino acid-15N profiles were determined.",

author = "Stein, {T. P.} and Leskiw, {M. J.} and Liquori, {E. M.} and Brooks, {H. B.} and Wallace, {H. W.} and Blakemore, {W. S.}",

note = "Funding Information: by USPHS Grant No. AM 16658.",

year = "1975",

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doi = "10.1016/0003-2697(75)90615-6",

language = "English (US)",

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pages = "481--488",

journal = "Analytical Biochemistry",

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publisher = "Academic Press Inc.",

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TY - JOUR

T1 - The use of optical emission spectroscopy for human 15N tracer studies

AU - Stein, T. P.

AU - Leskiw, M. J.

AU - Liquori, E. M.

AU - Brooks, H. B.

AU - Wallace, H. W.

AU - Blakemore, W. S.

N1 - Funding Information: by USPHS Grant No. AM 16658.

PY - 1975/6

Y1 - 1975/6

N2 - Optical emission spectroscopy is a convenient method for determing 15N specific activity in a variety of metabolites following the administration of an 15N-labeled amino acid to humans. The only disadvantage, compared to the more conventional isotope ratio mass spectrometer analytical system is that more tracer is needed to give accurate results. When an 15N-labeled amino acid is used as the 15N carrier, the amount of 15N is not above a tracer dose. A suitable procedure is described for performing such studies using [15N]glycine as a tracer. As an experimental example, [15N]glycine was infused into a subject at a rate of 35 mg of N/hr for 8 hr. After 3 hr a meal was consumed by the subject. The urinary urea-15N, ammonia-15N and amino acid-15N profiles were determined.

AB - Optical emission spectroscopy is a convenient method for determing 15N specific activity in a variety of metabolites following the administration of an 15N-labeled amino acid to humans. The only disadvantage, compared to the more conventional isotope ratio mass spectrometer analytical system is that more tracer is needed to give accurate results. When an 15N-labeled amino acid is used as the 15N carrier, the amount of 15N is not above a tracer dose. A suitable procedure is described for performing such studies using [15N]glycine as a tracer. As an experimental example, [15N]glycine was infused into a subject at a rate of 35 mg of N/hr for 8 hr. After 3 hr a meal was consumed by the subject. The urinary urea-15N, ammonia-15N and amino acid-15N profiles were determined.

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The use of optical emission spectroscopy for human ¹⁵N tracer studies

Abstract

All Science Journal Classification (ASJC) codes

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