The Sin3p PAH domains provide separate functions repressing meiotic gene transcription in Saccharomyces cerevisiae

Michael J. Mallory, Michael J. Law, Lela E. Buckingham, Randy Strich

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Meiotic genes in budding yeast are repressed during vegetative growth but are transiently induced during specific stages of meiosis. Sin3p represses the early meiotic gene (EMG) by bridging the DNA binding protein Ume6p to the histone deacetylase Rpd3p. Sin3p contains four paired amphipathic helix (PAH) domains, one of which (PAH3) is required for repressing several genes expressed during mitotic cell division. This report examines the roles of the PAH domains in mediating EMG repression during mitotic cell division and following meiotic induction. PAH2 and PAH3 are required for mitotic EMG repression, while electrophoretic mobility shift assays indicate that only PAH2 is required for stable Ume6p-promoter interaction. Unlike mitotic repression, reestablishing EMG repression following transient meiotic induction requires PAH3 and PAH4. In addition, the role of Sin3p in reestablishing repression is expanded to include additional loci that it does not control during vegetative growth. These findings indicate that mitotic and postinduction EMG repressions are mediated by two separate systems that utilize different Sin3p domains.

Original languageEnglish (US)
Pages (from-to)1835-1844
Number of pages10
JournalEukaryotic Cell
Volume9
Issue number12
DOIs
StatePublished - Dec 2010

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'The Sin3p PAH domains provide separate functions repressing meiotic gene transcription in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this