The mouse mammary tumor virus long terminal repeat encodes A 47 kDa Glycoprotein with a short half-life in mammalian cells

Claude Krummenacher, Heidi Diggelmann

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The mouse mammary tumor virus proviral DNA contains an open reading frame in the 3' long terminal repeat which can code for a 36 kDa polypeptide with a putative transmembrane sequence and five Nlinked glycosylation sites. This gene is known to code for a superantigen which deletes a specific subset of CD4+ T lymphocytes in vivo. The superantigen encoded by the exogenous mouse mammary tumor virus of the GR strain acts specifically on Vβ 14 bearing T cells. We produced recombinant vaccinia viruses to express either the complete or a truncated ORF protein after infection of primate cells in culture. The complete ORF gene in mammalian cells leads to the production of a 47 kDa protein which is specifically detected with an anti-ORF-peptide antiserum. The 47 kDa protein can be labeled with d-[2-3H]mannose and its synthesis is inhibited by tunicamycin, an N-linked glycosylation inhibitor, indicating that it is a glycoprotein. The truncated ORF protein beginning at the second ATG of the open reading frame is also modified, but the C-terminal half of ORF, starting at the fifth ATG, has the expected size of the non modified polypeptide. Pulse-chase experiments indicate that the ORF protein has a short half-life of about 1.5-2hr.

Original languageEnglish (US)
Pages (from-to)1151-1157
Number of pages7
JournalMolecular Immunology
Volume30
Issue number13
DOIs
StatePublished - Sep 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Fingerprint

Dive into the research topics of 'The mouse mammary tumor virus long terminal repeat encodes A 47 kDa Glycoprotein with a short half-life in mammalian cells'. Together they form a unique fingerprint.

Cite this