Abstract
The metabolism of leukotrienes (B4, C4, D4, and E4) within human plasma was studied and a simple sample preparation is presented. It was demonstrated that leukotriene E4 and leukotriene B4 were stable during incubation at 37° C using the in vitro system. In contrast, leukotriene C4 was metabolized by γ-glutamyl transpeptidase activities into leukotriene D4 which was further metabolized by dipeptidase activities of plasma into leukotriene E4. The transition state inhibitor of γ-glutamyl transpeptidase l-serine-borate decreased the metabolism of leukotriene C4 in plasma. Dilution of plasma demonstrated that the dipeptidase was more active compared to the γ-glutamyl transpeptidase. The metabolizing activities of plasma were functionally characterized by fractionating the plasma proteins.
Original language | English (US) |
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Pages (from-to) | 128-134 |
Number of pages | 7 |
Journal | BBA - Specialised Section On Lipids and Related Subjects |
Volume | 833 |
Issue number | 1 |
DOIs | |
State | Published - Jan 9 1985 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Endocrinology