The intrinsic fluorescence of the recombinant human leukocyte interferon-αA and fibroblast interferonβ1

S. I. Borukhov, A. Ya Strongin

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The environment of Trp residues of the recombinant human interferons has been studied by the analysis of the emission spectra of native and denatured proteins at pH 1,5-8,5 and temperature 10-65°C in the presence and absence of the anionic, cationic and neutral to charge contact quenchers-KI, CsCl and acrylamide, respectively. The obtained data allow to suppose that in IFN-αA and IFN-β1 Trp141 interacts with Arg145 and one or several from the following residues-Phe124, Ile127, Tyr130, Leu131, whereas Trp77 - with Arg33 and Phe36, Phe78, Leu81 or Leu82 (Ile81 or Val82 for IFN-β1).

Original languageEnglish (US)
Pages (from-to)282-288
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume169
Issue number1
DOIs
StatePublished - May 31 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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