The environment of Trp residues of the recombinant human interferons has been studied by the analysis of the emission spectra of native and denatured proteins at pH 1,5-8,5 and temperature 10-65°C in the presence and absence of the anionic, cationic and neutral to charge contact quenchers-KI, CsCl and acrylamide, respectively. The obtained data allow to suppose that in IFN-αA and IFN-β1 Trp141 interacts with Arg145 and one or several from the following residues-Phe124, Ile127, Tyr130, Leu131, whereas Trp77 - with Arg33 and Phe36, Phe78, Leu81 or Leu82 (Ile81 or Val82 for IFN-β1).
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - May 31 1990|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology