The effects of insulin on urinary urea and ammonia production

T. P. Stein, M. J. Leskiw, H. W. Wallace, W. S. Blakemore

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15N-Iabelled glycine, ammonium chloride, and L-aspartic acid were infused intravenously into a series of non-diabetic adults at a constant rate for 4 to 8 hours. Midway during the infusion, a pulse of 8-10 units of regular insulin was injected as a pulse or infused at a constant rate for the remainder of the15N infusion period. Frequent urine specimens were collected during the infusion period. The15N enrichment of the urinary urea, ammonia, and amino acids increased smoothly with time until the insulin was given. The pulse of insulin caused a downturn on the15N enrichment. We concluded from this that a) the effect of a pulse of insulin on human urea and ammonia metabolism lasted for about one hour, and b) insulin affected glutamine distribution in the plasma.

Original languageEnglish (US)
Pages (from-to)18-25
Number of pages8
JournalJournal of Clinical Endocrinology and Metabolism
Issue number1
StatePublished - Jan 1 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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