TY - JOUR
T1 - The effects of glucose and amino acids on tumor and host DNA synthesis
AU - Fried, Robert C.
AU - Mullen, James
AU - Stein, T. Peter
AU - Yuhas, John
AU - Miller, Elizabeth
AU - Buzby, Gordon P.
N1 - Funding Information:
’ Presented at the Annual Meeting of the Association for Academic Surgery, San Antonio, Texas, October 3 l-November 3, 1984. * This work was supported in part by NC1 Grant 5-T32-CA09430-0 1, VA Medical Research, Educational Grants from American McGaw Laboratories, the McCabe Fund, and the John Rhea Barton Foundation.
PY - 1985/11
Y1 - 1985/11
N2 - Tumor-bearing animals provided with intravenous glucose and amino acids (TPN) exhibit enhanced response to S-phase-specific chemotherapeutic agents (H. M. Reynolds, J. M. Daly, B. Rowlands, S. J. Dudrick, and E. M. Copeland. Cancer45: 3069, 1980; M. H. Torosian, J. L. Mullen, E. E. Miller, et al. J. Parenter. Enteral Nutr. 7: 337, 1983). To determine the mechanism of this response, DNA synthesis rate during starvation or a 48-hr infusion of glucose/amino acids (Glu/AA) was evaluated in tumor, liver, and terminal ileal cells of 68 rats. Tumor cells exhibited a rapid increase in DNA synthesis following the initiation of an infusion of Glu/AA. This increase was most marked after 2 hr of infusion and returned to control levels within 24 hr. Liver DNA synthesis rate increased in both starved and Glu/AA animals over 48 hr with a larger increase in animals receiving Glu/AA. Ileal DNA synthesis decreased equally in both groups. Short pulse Glu/AA produced transient increases in tumor DNA synthesis. Changes in host tissues occurred but followed a different temporal sequence. This may indicate the existence of a period of time following initiation of metabolic manipulation when tumor susceptibility to phase-specific chemotherapeutic agents will be enhanced while host tissues will be spared from increased toxicity.
AB - Tumor-bearing animals provided with intravenous glucose and amino acids (TPN) exhibit enhanced response to S-phase-specific chemotherapeutic agents (H. M. Reynolds, J. M. Daly, B. Rowlands, S. J. Dudrick, and E. M. Copeland. Cancer45: 3069, 1980; M. H. Torosian, J. L. Mullen, E. E. Miller, et al. J. Parenter. Enteral Nutr. 7: 337, 1983). To determine the mechanism of this response, DNA synthesis rate during starvation or a 48-hr infusion of glucose/amino acids (Glu/AA) was evaluated in tumor, liver, and terminal ileal cells of 68 rats. Tumor cells exhibited a rapid increase in DNA synthesis following the initiation of an infusion of Glu/AA. This increase was most marked after 2 hr of infusion and returned to control levels within 24 hr. Liver DNA synthesis rate increased in both starved and Glu/AA animals over 48 hr with a larger increase in animals receiving Glu/AA. Ileal DNA synthesis decreased equally in both groups. Short pulse Glu/AA produced transient increases in tumor DNA synthesis. Changes in host tissues occurred but followed a different temporal sequence. This may indicate the existence of a period of time following initiation of metabolic manipulation when tumor susceptibility to phase-specific chemotherapeutic agents will be enhanced while host tissues will be spared from increased toxicity.
UR - http://www.scopus.com/inward/record.url?scp=0022404358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022404358&partnerID=8YFLogxK
U2 - 10.1016/0022-4804(85)90101-5
DO - 10.1016/0022-4804(85)90101-5
M3 - Article
C2 - 3932782
AN - SCOPUS:0022404358
VL - 39
SP - 461
EP - 469
JO - Journal of Surgical Research
JF - Journal of Surgical Research
SN - 0022-4804
IS - 5
ER -