The effect of cellular differentiation on HSV-1 infection of oligodendrocytic cells

Raquel Bello-Morales, Antonio Jesús Crespillo, Beatriz García, Luis Ángel Dorado, Beatriz Martín, Enrique Tabarés, Claude Krummenacher, Fernando De Castro, José Antonio López-Guerrero

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


Herpes simplex type 1 (HSV-1) is a neurotropic virus that infects many types of cells. Previous studies have demonstrated that oligodendrocytic cells are highly susceptible to HSV-1 infection. Here we analysed HSV-1 infection of a human oligodendrocytic cell line, HOG, and oligodendrocyte precursor cells (OPCs) cultured under growth or differentiation conditions. In addition to cell susceptibility, the role of the major cell receptors for viral entry was assessed. Our results revealed that OPCs and HOG cells cultured under differentiation conditions became more susceptible to HSV-1. On the other hand, viral infection induced morphological changes corresponding to differentiated cells, suggesting that HSV-1 might be inducing cell differentiation. We also observed colocalization of HVEM and nectin-1 with viral particles, suggesting that these two major HSV-1 receptors are functional in HOG cells. Finally, electron microscopy assays indicated that HSV-1 may be also entering OLs by macropinocytosis depending on their differentiation stage. In addition, vesicles containing intracellular enveloped virions observed in differentiated cells point to an endocytic mechanism of virus entry. All these data are indicative of diverse entry pathways dependent on the maturation stage of OLs.

Original languageEnglish (US)
Article numbere89141
JournalPloS one
Issue number2
StatePublished - Feb 13 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General


Dive into the research topics of 'The effect of cellular differentiation on HSV-1 infection of oligodendrocytic cells'. Together they form a unique fingerprint.

Cite this