TY - JOUR
T1 - The conserved histone deacetylase Rpd3 and its DNA binding subunit Ume6 control dynamic transcript architecture during mitotic growth and meiotic development
AU - Lardenois, Aurelie
AU - Stuparevic, Igor
AU - Liu, Yuchen
AU - Law, Michael J.
AU - Becker, Emmanuelle
AU - Smagulova, Fatima
AU - Waern, Karl
AU - Guilleux, Marie Helene
AU - Horecka, Joe
AU - Chu, Angela
AU - Kervarrec, Christine
AU - Strich, Randy
AU - Snyder, Mike
AU - Davis, Ronald W.
AU - Steinmetz, Lars M.
AU - Primig, Michael
N1 - Publisher Copyright:
© 2014 The Author(s).
PY - 2015/9/1
Y1 - 2015/9/1
N2 - It was recently reported that the sizes of many mRNAs change when budding yeast cells exit mitosis and enter the meiotic differentiation pathway. These differences were attributed to length variations of their untranslated regions. The function of UTRs in protein translation is well established. However, the mechanism controlling the expression of distinct transcript isoforms during mitotic growth and meiotic development is unknown. In this study, we order developmentally regulated transcript isoforms according to their expression at specific stages during meiosis and gametogenesis, as compared to vegetative growth and starvation. We employ regulatory motif prediction, in vivo protein-DNA binding assays, genetic analyses and monitoring of epigenetic amino acid modification patterns to identify a novel role for Rpd3 and Ume6, two components of a histone deacetylase complex already known to repress early meiosis-specific genes in dividing cells, in mitotic repression of meiosis-specific transcript isoforms. Our findings classify developmental stage-specific early, middle and late meiotic transcript isoforms, and they point to a novel HDAC-dependent control mechanism for flexible transcript architecture during cell growth and differentiation. Since Rpd3 is highly conserved and ubiquitously expressed in many tissues, our results are likely relevant for development and disease in higher eukaryotes.
AB - It was recently reported that the sizes of many mRNAs change when budding yeast cells exit mitosis and enter the meiotic differentiation pathway. These differences were attributed to length variations of their untranslated regions. The function of UTRs in protein translation is well established. However, the mechanism controlling the expression of distinct transcript isoforms during mitotic growth and meiotic development is unknown. In this study, we order developmentally regulated transcript isoforms according to their expression at specific stages during meiosis and gametogenesis, as compared to vegetative growth and starvation. We employ regulatory motif prediction, in vivo protein-DNA binding assays, genetic analyses and monitoring of epigenetic amino acid modification patterns to identify a novel role for Rpd3 and Ume6, two components of a histone deacetylase complex already known to repress early meiosis-specific genes in dividing cells, in mitotic repression of meiosis-specific transcript isoforms. Our findings classify developmental stage-specific early, middle and late meiotic transcript isoforms, and they point to a novel HDAC-dependent control mechanism for flexible transcript architecture during cell growth and differentiation. Since Rpd3 is highly conserved and ubiquitously expressed in many tissues, our results are likely relevant for development and disease in higher eukaryotes.
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U2 - 10.1093/nar/gku1185
DO - 10.1093/nar/gku1185
M3 - Article
C2 - 25477386
AN - SCOPUS:84945197072
SN - 0305-1048
VL - 43
SP - 115
EP - 128
JO - Nucleic acids research
JF - Nucleic acids research
IS - 1
ER -