The C. elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable MicroRNA target

Shin Yi Lin, Steven M. Johnson, Mary Abraham, Monica C. Vella, Amy Pasquinelli, Chiara Gamberi, Ellen Gottlieb, Frank J. Slack

Research output: Contribution to journalArticle

268 Scopus citations

Abstract

Hunchback regulates the temporal identity of neuroblasts in Drosophila. Here we show that hbl-1, the C. elegans hunchback ortholog, also controls temporal patterning. Furthermore, hbl-1 is a probable target of microRNA regulation through its 3′UTR. hbl-1 loss-of-function causes the precocious expression of adult seam cell fates. This phenotype is similar to loss-of-function of lin-41, a known target of the let-7 microRNA. Like lin-41 mutations, hbl-1 loss-of-function partially suppresses a let-7 mutation. The hbl-1 3′UTR is both necessary and sufficient to downregulate a reporter gene during development, and the let-7 and lin-4 microRNAs are both required for HBL-1/GFP downregulation. Multiple elements in the hbl-1 3′UTR show complementarity to regulatory microRNAs, suggesting that microRNAs directly control hbl-1. MicroRNAs may likewise function to regulate Drosophila hunchback during temporal patterning of the nervous system.

Original languageEnglish (US)
Pages (from-to)639-650
Number of pages12
JournalDevelopmental Cell
Volume4
Issue number5
DOIs
StatePublished - May 1 2003

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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    Lin, S. Y., Johnson, S. M., Abraham, M., Vella, M. C., Pasquinelli, A., Gamberi, C., Gottlieb, E., & Slack, F. J. (2003). The C. elegans hunchback homolog, hbl-1, controls temporal patterning and is a probable MicroRNA target. Developmental Cell, 4(5), 639-650. https://doi.org/10.1016/S1534-5807(03)00124-2