The α(2D/A)-adrenergic receptor-linked membrane guanylate cyclase: A new signal transduction system in the pineal gland

Venkateswar Venkataraman, Teresa Duda, Rameshwar K. Sharma

Research output: Contribution to journalArticlepeer-review

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Abstract

In the pineal gland, the membrane guanylate cyclase activity was specifically stimulated by α(2D/A)-adrenergic receptor (α(2D/A)-AR) agonists. The agonists, however, did not stimulate the cyclase activity in the cell-free membranes. It was possible to stimulate the cyclase in cell-free membranes by the addition of the pineal soluble fraction, but this stimulation was Ca2+ dependent and α(2D/A)-agonist-independent. It was also possible to achieve Ca2+-dependent stimulation of the cyclase by the direct addition of CD-GCAP to the isolated pineal membranes. GD-GCAP is a Ca2+-binding protein and is a specific activator of one of the two membranes of the ROS-GC subfamily of membrane guanylate cyclases, ROS-GC1. The soluble fraction of the pineal gland stimulated recombinant ROS-GC1 in a Ca2+ dependent fashion. The direct presence of both ROS-GC1 and CD-GCAP in the pineal was established by molecular cloning/PCR studies. The findings demonstrate the existence of a novel signal transduction mechanism - the linkage of the α(2D/A)-AR signaling system with ROS-GC1 transduction system, occurring through intracellular Ca2+ via CD-GCAP.

Original languageEnglish (US)
Pages (from-to)69-73
Number of pages5
JournalFEBS Letters
Volume427
Issue number1
DOIs
StatePublished - May 1 1998

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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