TY - JOUR
T1 - Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy
AU - Taldone, Tony
AU - Gomes-Dagama, Erica M.
AU - Zong, Hongliang
AU - Sen, Siddhartha
AU - Alpaugh, Mary L.
AU - Zatorska, Danuta
AU - Alonso-Sabadell, Raul
AU - Guzman, Monica L.
AU - Chiosis, Gabriela
N1 - Funding Information:
This work was supported in part by W. H. Goodwin and A. Goodwin and the Commonwealth Cancer Foundation for Research, The Experimental Therapeutics Center of Memorial Sloan-Kettering Cancer Center (MSKCC), the Translational and Integrative Medicine Research Fund of MSKCC, SPORE Pilot Award and Research & Therapeutics Program in Prostate Cancer, Hirshberg Foundation for Pancreatic Cancer Research, 1R01CA155226-01, U01AG032969-01A1, R21AG028811, 3P30CA008748-44S2. G.C. is funded by Leukemia and Lymphoma Society LLS#6114-10 & LLS#6330-11, the Byrne Fund, the Geoffrey Beene Cancer Research Center of MSKCC, grant UL1RR024996 of the Clinical and Translational Science Center at Weill Cornell Medical College and Susan G. Komen for the Cure. T.T. is funded by Susan G. Komen for the Cure and the Department of Defense, Breast Cancer Research Program. M.L.G. is funded by the National Institutes of Health through the NIH Director’s New Innovator Award Program, 1 DP2 OD007399-01 and is a V-Foundation Scholar. We also thank Dr. George Sukenick and Dr. Hui Liu of the NMR Analytical Core Facility at MSKCC for expert mass spectral analysis.
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5] oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.
AB - Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5] oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.
UR - https://www.scopus.com/pages/publications/80051945455
UR - https://www.scopus.com/pages/publications/80051945455#tab=citedBy
U2 - 10.1016/j.bmcl.2011.07.026
DO - 10.1016/j.bmcl.2011.07.026
M3 - Article
C2 - 21802945
AN - SCOPUS:80051945455
SN - 0960-894X
VL - 21
SP - 5347
EP - 5352
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 18
ER -