Supercritical fluid chromatography- Nanospray ionization-mass spectrometry (SFC-nSI-MS)

Mahmoud Elhusseiny Mostafa, James P. Grinias, James L. Edwards

Research output: Contribution to journalArticlepeer-review

Abstract

A nanospray emitter coupled to a supercritical fluid chromatograph (SFC-nSI-MS) for mass spectrometric (MS) analysis of fatty acids (FA) positional isomers is introduced. The experimental setup uses conventional bore columns before the SF back-pressure regulator (pre-BPR). The flow is then split and nanosprayed using a short emitter post-BPR. A C18 column was used to resolve positional isomers of unsaturated FA with a 5 min gradient. Chromatographic resolution of the nSFC was compared to a LC-MS system with superior resolving power demonstrated in the nSFC MS system. This system has proven quantitative performance for analyzing pharmaceutical effects on FA composition in a complex biological matrix like E coli lysate.

Original languageEnglish (US)
Article number465377
JournalJournal of Chromatography A
Volume1736
DOIs
StatePublished - Nov 8 2024

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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