Studying transcription initiation by RNA polymerase with diffusion-based single-molecule fluorescence

Yazan Alhadid, Sang Yoon Chung, Eitan Lerner, Dylan J. Taatjes, Sergei Borukhov, Shimon Weiss

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Over the past decade, fluorescence-based single-molecule studies significantly contributed to characterizing the mechanism of RNA polymerase at different steps in transcription, especially in transcription initiation. Transcription by bacterial DNA-dependent RNA polymerase is a multistep process that uses genomic DNA to synthesize complementary RNA molecules. Transcription initiation is a highly regulated step in E. coli, but it has been challenging to study its mechanism because of its stochasticity and complexity. In this review, we describe how single-molecule approaches have contributed to our understanding of transcription and have uncovered mechanistic details that were not observed in conventional assays because of ensemble averaging.

Original languageEnglish (US)
Pages (from-to)1278-1290
Number of pages13
JournalProtein Science
Volume26
Issue number7
DOIs
StatePublished - Jul 1 2017

Fingerprint

DNA-Directed RNA Polymerases
Transcription
Fluorescence
Bacterial DNA
Complementary RNA
Molecules
Escherichia coli
DNA
Assays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Alhadid, Yazan ; Chung, Sang Yoon ; Lerner, Eitan ; Taatjes, Dylan J. ; Borukhov, Sergei ; Weiss, Shimon. / Studying transcription initiation by RNA polymerase with diffusion-based single-molecule fluorescence. In: Protein Science. 2017 ; Vol. 26, No. 7. pp. 1278-1290.
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Studying transcription initiation by RNA polymerase with diffusion-based single-molecule fluorescence. / Alhadid, Yazan; Chung, Sang Yoon; Lerner, Eitan; Taatjes, Dylan J.; Borukhov, Sergei; Weiss, Shimon.

In: Protein Science, Vol. 26, No. 7, 01.07.2017, p. 1278-1290.

Research output: Contribution to journalReview article

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AU - Chung, Sang Yoon

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AU - Borukhov, Sergei

AU - Weiss, Shimon

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