Studies on the migration of primordial germ cells in early chick embryos: Cultivation of hypoblast explants from the germinal crescent area

Hsin Yi Lee, John B. Schumann, Robert G. Nagele

Research output: Contribution to journalArticlepeer-review

Abstract

The hypoblast from the germinal crescent area of stage 4 to 5 chick embryos, when grown on a glass surface, spreads out as a thin and continuous cellular sheet. Primordial germ cells (PCCs) are found to migrate individually over this hypoblast monolayer (as they do in vivo) and, perhaps even more striking, their morphological features during migration are remarkably similar to those observed in vivo. Inasmuch as this in vitro system permits direct observations of PGCs migrating on their natural, rather than artificial, substratum undoubtedly it will prove to be an effective means of studying the mode of PGC migration in early chick embryos.

Original languageEnglish (US)
Pages (from-to)111-116
Number of pages6
JournalIn Vitro
Volume19
Issue number2
DOIs
StatePublished - Feb 1983

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science

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