Structure of unliganded HSV gD reveals a mechanism for receptor-mediated activation of virus entry

Claude Krummenacher, Vinit M. Supekar, J. Charles Whitbeck, Eric Lazear, Sarah A. Connolly, Roselyn J. Eisenberg, Gary H. Cohen, Don C. Wiley, Andrea Carfí

Research output: Contribution to journalArticlepeer-review

197 Scopus citations

Abstract

Herpes simplex virus (HSV) entry into cells requires binding of the envelope glycoprotein D (gD) to one of several cell surface receptors. The 50 C-terminal residues of the gD ectodomain are essential for virus entry, but not for receptor binding. We have determined the structure of an unliganded gD molecule that includes these C-terminal residues. The structure reveals that the C-terminus is anchored near the N-terminal region and masks receptor-binding sites. Locking the C-terminus in the position observed in the crystals by an intramolecular disulfide bond abolished receptor binding and virus entry, demonstrating that this region of gD moves upon receptor binding. Similarly, a point mutant that would destabilize the C-terminus structure was nonfunctional for entry, despite increased affinity for receptors. We propose that a controlled displacement of the gD C-terminus upon receptor binding is an essential feature of HSV entry, ensuring the timely activation of membrane fusion.

Original languageEnglish (US)
Pages (from-to)4144-4153
Number of pages10
JournalEMBO Journal
Volume24
Issue number23
DOIs
StatePublished - Dec 7 2005

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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