TY - GEN
T1 - Specific targeting and delivery of virus envelope-coated nanoparticle cargoes into receptor-bearing cells and subcellular compartments
AU - Moore, A.
AU - Kolokoltsov, A.
AU - Boehning, D.
AU - Albrecht, T.
AU - Davey, R.
PY - 2007
Y1 - 2007
N2 - Viruses are highly efficient nanoscale machines that not only target specific cell types but also penetrate the cell membrane to deliver the virus genome into the cytoplasm. Recently, we described a simple and highly scalable method to modify the surface of nanoparticles with the membranes of murine leukemia viruses (MLV) and showed that these can deliver a simple cargo into receptor bearing cells (Deniger et al., Nano Lett. 2006 Nov;6(l 1):2414-21). However, the entry route and subcellular destination of the nanocargoes was not determined. Here we use state-of-the-art microscopy techniques on live cells to visualize the penetration and delivery of the cargo to different subcellular compartments. We show that the MLV membrane-coated nanoparticles are specifically captured on the surface of receptor bearing cells from which they are rapidly taken up into early endosomes. We predict that coating of nanoparticle cargoes with virus envelopes will create a biologically compatible capsule to enhance circulation and delivery of nanoscale drug formulations to specific cells and subcellular compartments.
AB - Viruses are highly efficient nanoscale machines that not only target specific cell types but also penetrate the cell membrane to deliver the virus genome into the cytoplasm. Recently, we described a simple and highly scalable method to modify the surface of nanoparticles with the membranes of murine leukemia viruses (MLV) and showed that these can deliver a simple cargo into receptor bearing cells (Deniger et al., Nano Lett. 2006 Nov;6(l 1):2414-21). However, the entry route and subcellular destination of the nanocargoes was not determined. Here we use state-of-the-art microscopy techniques on live cells to visualize the penetration and delivery of the cargo to different subcellular compartments. We show that the MLV membrane-coated nanoparticles are specifically captured on the surface of receptor bearing cells from which they are rapidly taken up into early endosomes. We predict that coating of nanoparticle cargoes with virus envelopes will create a biologically compatible capsule to enhance circulation and delivery of nanoscale drug formulations to specific cells and subcellular compartments.
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M3 - Conference contribution
AN - SCOPUS:34547996982
SN - 1420063421
SN - 9781420063424
SN - 1420061836
SN - 9781420061833
T3 - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
SP - 370
EP - 373
BT - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007, Technical Proceedings
T2 - 2007 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2007
Y2 - 20 May 2007 through 24 May 2007
ER -