TY - JOUR
T1 - SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae
AU - Krisak, Lisa
AU - Strich, Randy
AU - Winters, R. Scott
AU - Hall, J. Perry
AU - Mallory, Michael J.
AU - Kreitzer, David
AU - Tuan, Rocky S.
AU - Winter, Edward
PY - 1994/9/15
Y1 - 1994/9/15
N2 - Mitogen-activated protein (MAP) kinases comprise a family of conserved, eukaryotic enzymes that mediate responses to a wide variety of extracellular stimuli. In yeast, different signal transduction pathways utilize distinct MAP kinase family members. We have identified a new yeast MAP kinase gene (named SMK1) that is required for the completion of sporulation. Molecular and cytologic markers indicate that meiotic development proceeds normally in homozygous smk1-Δ1 diploids through meiosis II. However, light and electron microscopy show that smk1 asci are defective in organizing spore wall assembly. Consistent with a defect in spore wall assembly, smk1-Δ1 mutant asci display enhanced sensitivities to enzymatic digestion, heat shock, and exposure to ether. SMK1 mRNA, which is not detectable in vegetative cells, is derepressed at least 200-fold just prior to prospore enclosure. We propose that the SMK1 MAP kinase participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.
AB - Mitogen-activated protein (MAP) kinases comprise a family of conserved, eukaryotic enzymes that mediate responses to a wide variety of extracellular stimuli. In yeast, different signal transduction pathways utilize distinct MAP kinase family members. We have identified a new yeast MAP kinase gene (named SMK1) that is required for the completion of sporulation. Molecular and cytologic markers indicate that meiotic development proceeds normally in homozygous smk1-Δ1 diploids through meiosis II. However, light and electron microscopy show that smk1 asci are defective in organizing spore wall assembly. Consistent with a defect in spore wall assembly, smk1-Δ1 mutant asci display enhanced sensitivities to enzymatic digestion, heat shock, and exposure to ether. SMK1 mRNA, which is not detectable in vegetative cells, is derepressed at least 200-fold just prior to prospore enclosure. We propose that the SMK1 MAP kinase participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.
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U2 - 10.1101/gad.8.18.2151
DO - 10.1101/gad.8.18.2151
M3 - Article
C2 - 7958885
AN - SCOPUS:0027967087
VL - 8
SP - 2151
EP - 2161
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 18
ER -