SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae

Lisa Krisak, Randy Strich, R. Scott Winters, J. Perry Hall, Michael J. Mallory, David Kreitzer, Rocky S. Tuan, Edward Winter

Research output: Contribution to journalArticlepeer-review

139 Scopus citations

Abstract

Mitogen-activated protein (MAP) kinases comprise a family of conserved, eukaryotic enzymes that mediate responses to a wide variety of extracellular stimuli. In yeast, different signal transduction pathways utilize distinct MAP kinase family members. We have identified a new yeast MAP kinase gene (named SMK1) that is required for the completion of sporulation. Molecular and cytologic markers indicate that meiotic development proceeds normally in homozygous smk1-Δ1 diploids through meiosis II. However, light and electron microscopy show that smk1 asci are defective in organizing spore wall assembly. Consistent with a defect in spore wall assembly, smk1-Δ1 mutant asci display enhanced sensitivities to enzymatic digestion, heat shock, and exposure to ether. SMK1 mRNA, which is not detectable in vegetative cells, is derepressed at least 200-fold just prior to prospore enclosure. We propose that the SMK1 MAP kinase participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.

Original languageEnglish (US)
Pages (from-to)2151-2161
Number of pages11
JournalGenes and Development
Volume8
Issue number18
DOIs
StatePublished - Sep 15 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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