Selective Regulation of Arterial Branching Morphogenesis by Synectin

Thomas W. Chittenden; Filip Claes; Anthony A. Lanahan; Monica Autiero; Robert T. Palac; Eugene V. Tkachenko; Arye Elfenbein; Carmen Ruiz de Almodovar; Eduard Dedkov; Robert Tomanek; Weiming Li; Michael Westmore; Jai Pal Singh; Arie Horowitz; Mary Jo Mulligan-Kehoe; Karen L. Moodie; Zhen W. Zhuang; Peter Carmeliet; Michael Simons

doi:10.1016/j.devcel.2006.03.012

Selective Regulation of Arterial Branching Morphogenesis by Synectin

Thomas W. Chittenden, Filip Claes, Anthony A. Lanahan, Monica Autiero, Robert T. Palac, Eugene V. Tkachenko, Arye Elfenbein, Carmen Ruiz de Almodovar, Eduard Dedkov, Robert Tomanek, Weiming Li, Michael Westmore, Jai Pal Singh, Arie Horowitz, Mary Jo Mulligan-Kehoe, Karen L. Moodie, Zhen W. Zhuang, Peter Carmeliet, Michael Simons

Research output: Contribution to journal › Article › peer-review

120 Scopus citations

Abstract

Branching morphogenesis is a key process in the formation of vascular networks. To date, little is known regarding the molecular events regulating this process. We investigated the involvement of synectin in this process. In zebrafish embryos, synectin knockdown resulted in a hypoplastic dorsal aorta and hypobranched, stunted, and thin intersomitic vessels due to impaired migration and proliferation of angioblasts and arterial endothelial cells while not affecting venous development. Synectin^-/- mice demonstrated decreased body and organ size, reduced numbers of arteries, and an altered pattern of arterial branching in multiple vascular beds while the venous system remained normal. Murine synectin^-/- primary arterial, but not venous, endothelial cells showed decreased in vitro tube formation, migration, and proliferation and impaired polarization due to abnormal localization of activated Rac1. We conclude that synectin is involved in selective regulation of arterial, but not venous, growth and branching morphogenesis and that Rac1 plays an important role in this process.

Original language	English (US)
Pages (from-to)	783-795
Number of pages	13
Journal	Developmental Cell
Volume	10
Issue number	6
DOIs	https://doi.org/10.1016/j.devcel.2006.03.012
State	Published - Jun 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
General Biochemistry, Genetics and Molecular Biology
Developmental Biology
Cell Biology

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10.1016/j.devcel.2006.03.012

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Chittenden, T. W., Claes, F., Lanahan, A. A., Autiero, M., Palac, R. T., Tkachenko, E. V., Elfenbein, A., Ruiz de Almodovar, C., Dedkov, E., Tomanek, R., Li, W., Westmore, M., Singh, J. P., Horowitz, A., Mulligan-Kehoe, M. J., Moodie, K. L., Zhuang, Z. W., Carmeliet, P., & Simons, M. (2006). Selective Regulation of Arterial Branching Morphogenesis by Synectin. Developmental Cell, 10(6), 783-795. https://doi.org/10.1016/j.devcel.2006.03.012

@article{4ebfe2e3600e47b4a088ad083be362dc,

title = "Selective Regulation of Arterial Branching Morphogenesis by Synectin",

abstract = "Branching morphogenesis is a key process in the formation of vascular networks. To date, little is known regarding the molecular events regulating this process. We investigated the involvement of synectin in this process. In zebrafish embryos, synectin knockdown resulted in a hypoplastic dorsal aorta and hypobranched, stunted, and thin intersomitic vessels due to impaired migration and proliferation of angioblasts and arterial endothelial cells while not affecting venous development. Synectin-/- mice demonstrated decreased body and organ size, reduced numbers of arteries, and an altered pattern of arterial branching in multiple vascular beds while the venous system remained normal. Murine synectin-/- primary arterial, but not venous, endothelial cells showed decreased in vitro tube formation, migration, and proliferation and impaired polarization due to abnormal localization of activated Rac1. We conclude that synectin is involved in selective regulation of arterial, but not venous, growth and branching morphogenesis and that Rac1 plays an important role in this process.",

author = "Chittenden, {Thomas W.} and Filip Claes and Lanahan, {Anthony A.} and Monica Autiero and Palac, {Robert T.} and Tkachenko, {Eugene V.} and Arye Elfenbein and {Ruiz de Almodovar}, Carmen and Eduard Dedkov and Robert Tomanek and Weiming Li and Michael Westmore and Singh, {Jai Pal} and Arie Horowitz and Mulligan-Kehoe, {Mary Jo} and Moodie, {Karen L.} and Zhuang, {Zhen W.} and Peter Carmeliet and Michael Simons",

year = "2006",

month = jun,

doi = "10.1016/j.devcel.2006.03.012",

language = "English (US)",

volume = "10",

pages = "783--795",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "6",

}

Chittenden, TW, Claes, F, Lanahan, AA, Autiero, M, Palac, RT, Tkachenko, EV, Elfenbein, A, Ruiz de Almodovar, C, Dedkov, E, Tomanek, R, Li, W, Westmore, M, Singh, JP, Horowitz, A, Mulligan-Kehoe, MJ, Moodie, KL, Zhuang, ZW, Carmeliet, P & Simons, M 2006, 'Selective Regulation of Arterial Branching Morphogenesis by Synectin', Developmental Cell, vol. 10, no. 6, pp. 783-795. https://doi.org/10.1016/j.devcel.2006.03.012

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T1 - Selective Regulation of Arterial Branching Morphogenesis by Synectin

AU - Chittenden, Thomas W.

AU - Claes, Filip

AU - Lanahan, Anthony A.

AU - Autiero, Monica

AU - Palac, Robert T.

AU - Tkachenko, Eugene V.

AU - Elfenbein, Arye

AU - Ruiz de Almodovar, Carmen

AU - Dedkov, Eduard

AU - Tomanek, Robert

AU - Li, Weiming

AU - Westmore, Michael

AU - Singh, Jai Pal

AU - Horowitz, Arie

AU - Mulligan-Kehoe, Mary Jo

AU - Moodie, Karen L.

AU - Zhuang, Zhen W.

AU - Carmeliet, Peter

AU - Simons, Michael

PY - 2006/6

Y1 - 2006/6

N2 - Branching morphogenesis is a key process in the formation of vascular networks. To date, little is known regarding the molecular events regulating this process. We investigated the involvement of synectin in this process. In zebrafish embryos, synectin knockdown resulted in a hypoplastic dorsal aorta and hypobranched, stunted, and thin intersomitic vessels due to impaired migration and proliferation of angioblasts and arterial endothelial cells while not affecting venous development. Synectin-/- mice demonstrated decreased body and organ size, reduced numbers of arteries, and an altered pattern of arterial branching in multiple vascular beds while the venous system remained normal. Murine synectin-/- primary arterial, but not venous, endothelial cells showed decreased in vitro tube formation, migration, and proliferation and impaired polarization due to abnormal localization of activated Rac1. We conclude that synectin is involved in selective regulation of arterial, but not venous, growth and branching morphogenesis and that Rac1 plays an important role in this process.

AB - Branching morphogenesis is a key process in the formation of vascular networks. To date, little is known regarding the molecular events regulating this process. We investigated the involvement of synectin in this process. In zebrafish embryos, synectin knockdown resulted in a hypoplastic dorsal aorta and hypobranched, stunted, and thin intersomitic vessels due to impaired migration and proliferation of angioblasts and arterial endothelial cells while not affecting venous development. Synectin-/- mice demonstrated decreased body and organ size, reduced numbers of arteries, and an altered pattern of arterial branching in multiple vascular beds while the venous system remained normal. Murine synectin-/- primary arterial, but not venous, endothelial cells showed decreased in vitro tube formation, migration, and proliferation and impaired polarization due to abnormal localization of activated Rac1. We conclude that synectin is involved in selective regulation of arterial, but not venous, growth and branching morphogenesis and that Rac1 plays an important role in this process.

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EP - 795

JO - Developmental Cell

JF - Developmental Cell

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ER -

Selective Regulation of Arterial Branching Morphogenesis by Synectin

Abstract

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