Role of proteolipid protein in HSV-1 entry in oligodendrocytic cells

Raquel Bello-Morales, Antonio Jesús Crespillo, Beatriz Praena, Enrique Tabarés, Yolanda Revilla, Elena García, Alberto Fraile-Ramos, Wia Baron, Claude Krummenacher, José Antonio López-Guerrero

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Herpes simplex virus type 1 (HSV-1) has the ability to enter many different hosts and cell types by several strategies. This highly prevalent alphaherpesvirus can enter target cells using different receptors and different pathways: fusion at a neutral pH, low-pH-dependent and low-pH-independent endocytosis. Several cell receptors for viral entry have been described, but several observations suggest that more receptors for HSV-1 might exist. In this work, we propose a novel role for the proteolipid protein (PLP) in HSV-1 entry into the human oligodendrocytic cell line HOG. Cells transfected with PLP-EGFP showed an increase in susceptibility to HSV-1. Furthermore, the infection of HOG and HOG-PLP transfected cells with the R120vGF virus-unable to replicate in ICP4-defficient cells-showed an increase in viral signal in HOG-PLP, suggesting a PLP involvement in viral entry. In addition, a mouse monoclonal antibody against PLP drastically inhibited HSV-1 entry into HOG cells. PLP and virions colocalized in confocal immunofluorescence images, and in electron microscopy images, which suggest that PLP acts at the site of entry into HOG cells. Taken together these results suggest that PLP may be involved in HSV-1 entry in human oligodendrocytic cells.

Original languageEnglish (US)
Article numbere0147885
JournalPloS one
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2016

All Science Journal Classification (ASJC) codes

  • General

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