RNA polymerase holoenzyme: Structure, function and biological implications

Sergei Borukhov, Evgeny Nudler

Research output: Contribution to journalReview articlepeer-review

108 Scopus citations


The past three years have marked the breakthrough in our understanding of the structural and functional organization of RNA polymerase. The latest major advance was the high-resolution structures of bacterial RNA polymerase holoenzyme and the holoenzyme in complex with promoter DNA. Together with an array of genetic, biochemical and biophysical data accumulated to date, the structures provide a comprehensive view of dynamic interactions between the major components of transcription machinery during the early stages of the transcription cycle. They include the binding of sigma factor to the core enzyme, and the recognition of promoter sequences and DNA melting by holoenzyme, transcription initiation and promoter clearance.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalCurrent Opinion in Microbiology
Issue number2
StatePublished - Apr 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases


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