TY - JOUR
T1 - Relative rates of tumor, normal gut, liver, and fibrinogen protein synthesis in man
AU - Stein, T. P.
AU - Mullen, J. L.
AU - Oram-Smith, J. C.
PY - 1978
Y1 - 1978
N2 - Fractional protein synthesis rates of tumor, normal intestinal tissue, liver and fibrinogen were measured in a series of patients with gastrointestinal malignancies. Protein synthesis rates were measured by the continuous infusion of 95+% [15N]glycine. Twelve to eighteen hours prior to the clinically indicated surgical excision of the tumor, 1-2 g of 95+% [15N]glycine was infused at a constant rate into each subject. During the surgical procedure, 0.05-2 g of tumor, normal intestinal tissue, liver and 30 ml of venous blood were obtained. Protein synthesis rates were estimated from the ratio of 15N incorporated into tissue protein to the 15N enrichment of the tissue-free amino acid pool. The major findings were: i) the 15N enrichment of the tissue-free amino acids in malignant tissue was greater than and proportional to that in the corresponding normal tissue (P < 0.02); ii) tumor protein synthesis rates were greater than and proportional to the corresponding intestinal tissue rates (P < 0.05); iii) the fibrinogen synthesis rate was greater than the liver protein synthesis rate (P < 0.01), but there was no correlation between them.
AB - Fractional protein synthesis rates of tumor, normal intestinal tissue, liver and fibrinogen were measured in a series of patients with gastrointestinal malignancies. Protein synthesis rates were measured by the continuous infusion of 95+% [15N]glycine. Twelve to eighteen hours prior to the clinically indicated surgical excision of the tumor, 1-2 g of 95+% [15N]glycine was infused at a constant rate into each subject. During the surgical procedure, 0.05-2 g of tumor, normal intestinal tissue, liver and 30 ml of venous blood were obtained. Protein synthesis rates were estimated from the ratio of 15N incorporated into tissue protein to the 15N enrichment of the tissue-free amino acid pool. The major findings were: i) the 15N enrichment of the tissue-free amino acids in malignant tissue was greater than and proportional to that in the corresponding normal tissue (P < 0.02); ii) tumor protein synthesis rates were greater than and proportional to the corresponding intestinal tissue rates (P < 0.05); iii) the fibrinogen synthesis rate was greater than the liver protein synthesis rate (P < 0.01), but there was no correlation between them.
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U2 - 10.1152/ajpendo.1978.234.6.e648
DO - 10.1152/ajpendo.1978.234.6.e648
M3 - Article
C2 - 665769
AN - SCOPUS:0017978549
SN - 0363-6100
VL - 3
SP - E648-E652
JO - American Journal of Physiology Endocrinology Metabolism and Gastrointestinal Physiology
JF - American Journal of Physiology Endocrinology Metabolism and Gastrointestinal Physiology
IS - 6
ER -