Denatured tubulins form stable complexes with groEL upon dilution into refolding buffer. These complexes are retained on an immunoaffmity column which contains chemically immobilized antibodies to groEL. Tubulin remains bound to the immobilized groEL colunm after extensive washing and is released upon incubation with groES and ATP. Similar results were obtained with glutamine synthetase. These data suggest that groEL can function while it is attached to a solid support system.
|Original language||English (US)|
|Number of pages||4|
|Journal||Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular|
|State||Published - Sep 21 1994|
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology