Redox protein-polymer films for simultaneous determination of ascorbic acid and hydrogen peroxide

Amos Mugweru, Hoang Khoa, Emmanuel Yawson, Sagar Tolani, Adam Wanekaya

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Layer by layer films of protein and redox polymer were constructed and used to simultaneously analyze ascorbic acid and hydrogen peroxide. The films were made using hemoglobin and poly[4-vinylpyridine Os(bipyridine) 2 Cl]-co-ethylamine (Pos-Ea). The film growth was monitored using cyclic voltammetry, quartz crystal microbalance (QCM) and atomic force microscopy (AFM). Reversible pairs of oxidation-reduction peaks were observed using cyclic voltammetry corresponding to the Os II /Os III from redox polymer and HbFe III /HbFe II redox couples at 0.35 and -0.25 V vs. Ag/AgCl, respectively. The two redox centers were independent of each other. This enabled the simultaneous and independent determination of ascorbic acid and hydrogen. Peak currents were linearly related to concentration for both analytes in a mixture. The linear range of ascorbic acid was 0 - 1 mM (R 2 = 0.9996, n = 5) at scan rate of 50 mV s -1 (sensitivity 3.5 μA/mM) while hydrogen peroxide linear range was 1.0 - 10.0 μM (R 2 = 0.991, n = 6) with sensitivity of 1.85 μA/μM. 2008

Original languageEnglish (US)
Pages (from-to)1105-1110
Number of pages6
JournalAnalytical Sciences
Volume24
Issue number9
DOIs
StatePublished - Sep 2008

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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