Recombination induces tandem repeats of integrated viral sequences in polyoma-transformed cells

Karen Friderici; Saw Yin Oh; Ron Ellis; Vincent Guacci; Michele M. Fluck

doi:10.1016/0042-6822(84)90009-6

Recombination induces tandem repeats of integrated viral sequences in polyoma-transformed cells

Karen Friderici, Saw Yin Oh, Ron Ellis, Vincent Guacci, Michele M. Fluck

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Transformed Fischer rat cells derived from coinfections with wild-type polyoma virus and transformation-deficient hr-t deletion mutants were studied. The structure of the integrated viral DNA was determined by Southern blot analysis of high-molecular-weight DNA. In several cases the two parental genomes were found cointegrated in a head-totail manner at a single site in the host DNA. The results indicate that parental viral genomes recombine in Fischer rat cells in the process of transformation, and that the commonly observed tandem integration of viral genomes in polyoma-transformed cells must be due, in part, to recombination events.

Original language	English (US)
Pages (from-to)	67-73
Number of pages	7
Journal	Virology
Volume	137
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(84)90009-6
State	Published - Aug 1984
Externally published	Yes

All Science Journal Classification (ASJC) codes

Virology

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10.1016/0042-6822(84)90009-6

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title = "Recombination induces tandem repeats of integrated viral sequences in polyoma-transformed cells",

abstract = "Transformed Fischer rat cells derived from coinfections with wild-type polyoma virus and transformation-deficient hr-t deletion mutants were studied. The structure of the integrated viral DNA was determined by Southern blot analysis of high-molecular-weight DNA. In several cases the two parental genomes were found cointegrated in a head-totail manner at a single site in the host DNA. The results indicate that parental viral genomes recombine in Fischer rat cells in the process of transformation, and that the commonly observed tandem integration of viral genomes in polyoma-transformed cells must be due, in part, to recombination events.",

author = "Karen Friderici and {Yin Oh}, Saw and Ron Ellis and Vincent Guacci and Fluck, {Michele M.}",

note = "Funding Information: The excellent technical assistance of Claudette Priehs is gratefully acknowledged. The authors thank the departmental secretarial service. This work was supported by Grant CA 29270 from the National Cancer Institute. M.F. is a scholar from the Leukemia Society of America, Inc.",

year = "1984",

month = aug,

doi = "10.1016/0042-6822(84)90009-6",

language = "English (US)",

volume = "137",

pages = "67--73",

journal = "Virology",

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publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Recombination induces tandem repeats of integrated viral sequences in polyoma-transformed cells

AU - Friderici, Karen

AU - Yin Oh, Saw

AU - Ellis, Ron

AU - Guacci, Vincent

AU - Fluck, Michele M.

N1 - Funding Information: The excellent technical assistance of Claudette Priehs is gratefully acknowledged. The authors thank the departmental secretarial service. This work was supported by Grant CA 29270 from the National Cancer Institute. M.F. is a scholar from the Leukemia Society of America, Inc.

PY - 1984/8

Y1 - 1984/8

N2 - Transformed Fischer rat cells derived from coinfections with wild-type polyoma virus and transformation-deficient hr-t deletion mutants were studied. The structure of the integrated viral DNA was determined by Southern blot analysis of high-molecular-weight DNA. In several cases the two parental genomes were found cointegrated in a head-totail manner at a single site in the host DNA. The results indicate that parental viral genomes recombine in Fischer rat cells in the process of transformation, and that the commonly observed tandem integration of viral genomes in polyoma-transformed cells must be due, in part, to recombination events.

AB - Transformed Fischer rat cells derived from coinfections with wild-type polyoma virus and transformation-deficient hr-t deletion mutants were studied. The structure of the integrated viral DNA was determined by Southern blot analysis of high-molecular-weight DNA. In several cases the two parental genomes were found cointegrated in a head-totail manner at a single site in the host DNA. The results indicate that parental viral genomes recombine in Fischer rat cells in the process of transformation, and that the commonly observed tandem integration of viral genomes in polyoma-transformed cells must be due, in part, to recombination events.

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JO - Virology

JF - Virology

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ER -

Recombination induces tandem repeats of integrated viral sequences in polyoma-transformed cells

Abstract

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