Recognition of peptidyl epitopes by polymorphic epithelial mucin (PEM)- specific monoclonal antibodies

A. S. Dion, N. I. Smorodinsky, C. J. Williams, D. H. Wreschner, P. P. Major, I. Keydar

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Abstract

Peptidyl epitope recognition by several murine monoclonal antibodies (MAbs E29, H23, HMFG-1, HMFG-2, MA5, MA6 and MA9) which react with the polymorphic epithelial mucins [PEM; epithelial membrane antigen (EMA)] was studied by using ten synthetic peptides representative of the 20 residue tandem repeat as test antigens. Antibody binding to 6-10 residue overlaps and to peptides having a common carboxy-terminus and staggered amino-termini (8-31 residues) was assessed by solid phase and competition ELISA techniques. From these analyses, all MAbs except MA9 were found to react predominantly with the carboxy-terminal half of the repeat motif. Polyclonal antibody responses in mice immunized with intact EMA/PEM-containing preparations also displayed significant reactivities against synthetic repeat peptide antigens and, conversely, synthetic peptides as carrier-conjugated immunogens induced antibodies recognizing intact antigens. These results are discussed vis-a-vis peptide conformation, the potential effects of O-glycosylation on secondary structure, and the possible effects of these parameters on immunogenicity and antigenicity.

Original languageEnglish (US)
Pages (from-to)595-610
Number of pages16
JournalHybridoma
Volume10
Issue number5
DOIs
StatePublished - Jan 1 1991

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All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Dion, A. S., Smorodinsky, N. I., Williams, C. J., Wreschner, D. H., Major, P. P., & Keydar, I. (1991). Recognition of peptidyl epitopes by polymorphic epithelial mucin (PEM)- specific monoclonal antibodies. Hybridoma, 10(5), 595-610. https://doi.org/10.1089/hyb.1991.10.595