Recent advances in capillary ultrahigh pressure liquid chromatography

Laura E. Blue, Edward G. Franklin, Justin M. Godinho, James P. Grinias, Kaitlin M. Grinias, Daniel B. Lunn, Stephanie M. Moore

Research output: Contribution to journalReview articlepeer-review

48 Scopus citations

Abstract

In the twenty years since its initial demonstration, capillary ultrahigh pressure liquid chromatography (UHPLC) has proven to be one of most powerful separation techniques for the analysis of complex mixtures. This review focuses on the most recent advances made since 2010 towards increasing the performance of such separations. Improvements in capillary column preparation techniques that have led to columns with unprecedented performance are described. New stationary phases and phase supports that have been reported over the past decade are detailed, with a focus on their use in capillary formats. A discussion on the instrument developments that have been required to ensure that extra-column effects do not diminish the intrinsic efficiency of these columns during analysis is also included. Finally, the impact of these capillary UHPLC topics on the field of proteomics and ways in which capillary UHPLC may continue to be applied to the separation of complex samples are addressed.

Original languageEnglish (US)
Pages (from-to)17-39
Number of pages23
JournalJournal of Chromatography A
Volume1523
DOIs
StatePublished - Nov 10 2017

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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