Rapid creation of forward-genetics tools for C. briggsae Using TALENs: Lessons for nonmodel organisms

Qing Wei, Yongquan Shen, Xiangmei Chen, Yelena Shifman, Ronald E. Ellis

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Although evolutionary studies of gene function often rely on RNA interference, the ideal approach would use reverse genetics to create null mutations for cross-species comparisons and forward genetics to identify novel genes in each species. We have used transcription activator-like effector nucleases (TALENs) to facilitate both approaches in Caenorhabditis nematodes. First, by combining golden gate cloning and TALEN technology, we can induce frameshifting mutations in any gene. Second, by combining this approach with bioinformatics we can predict and create the resources needed for forward genetic analysis in species like Caenorhabditis briggsae. Although developing genetic model organisms used to require years to isolate marker mutations, balancers, and tools, with TALENs, these reagents can now be produced in months. Furthermore, the analysis of nonsense mutants in related model organisms allows a directed approach for making these markers and tools. When used together, these methods could simplify the adaptation of other organisms for forward and reverse genetics.

Original languageEnglish (US)
Pages (from-to)468-473
Number of pages6
JournalMolecular Biology and Evolution
Volume31
Issue number2
DOIs
StatePublished - Feb 2014

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Rapid creation of forward-genetics tools for C. briggsae Using TALENs: Lessons for nonmodel organisms'. Together they form a unique fingerprint.

Cite this