TY - JOUR
T1 - Proteomic study and marker protein identification of Caenorhabditis elegans lipid droplets
AU - Zhang, Peng
AU - Na, Huimin
AU - Liu, Zhenglong
AU - Zhang, Shuyan
AU - Xue, Peng
AU - Chen, Yong
AU - Pu, Jing
AU - Peng, Gong
AU - Huang, Xun
AU - Yang, Fuquan
AU - Xie, Zhensheng
AU - Xu, Tao
AU - Xu, Pingyong
AU - Ou, Guangshuo
AU - Zhang, Shaobing O.
AU - Liu, Pingsheng
PY - 2012/8
Y1 - 2012/8
N2 - Lipid droplets (LDs) are a neutral lipid storage organelle that is conserved across almost all species. Many metabolic syndromes are directly linked to the over-storage of neutral lipids in LDs. The study of LDs in Caenorhabditis elegans (C. elegans) has been difficult because of the lack of specific LD marker proteins. Here we report the purification and proteomic analysis of C. elegans lipid droplets for the first time. We identified 306 proteins, 63% of these proteins were previously known to be LD-proteins, suggesting a similarity between mammalian and C. elegans LDs. Using morphological and biochemical analyses, we show that short-chain dehydrogenase, DHS-3 is almost exclusively localized on C. elegans LDs, indicating that it can be used as a LD marker protein in C. elegans. These results will facilitate further mechanistic studies of LDs in this powerful genetic system, C. elegans.
AB - Lipid droplets (LDs) are a neutral lipid storage organelle that is conserved across almost all species. Many metabolic syndromes are directly linked to the over-storage of neutral lipids in LDs. The study of LDs in Caenorhabditis elegans (C. elegans) has been difficult because of the lack of specific LD marker proteins. Here we report the purification and proteomic analysis of C. elegans lipid droplets for the first time. We identified 306 proteins, 63% of these proteins were previously known to be LD-proteins, suggesting a similarity between mammalian and C. elegans LDs. Using morphological and biochemical analyses, we show that short-chain dehydrogenase, DHS-3 is almost exclusively localized on C. elegans LDs, indicating that it can be used as a LD marker protein in C. elegans. These results will facilitate further mechanistic studies of LDs in this powerful genetic system, C. elegans.
UR - http://www.scopus.com/inward/record.url?scp=84864818294&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864818294&partnerID=8YFLogxK
U2 - 10.1074/mcp.M111.016345
DO - 10.1074/mcp.M111.016345
M3 - Article
C2 - 22493183
AN - SCOPUS:84864818294
SN - 1535-9476
VL - 11
SP - 317
EP - 328
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 8
ER -