Cryostat sections of clinicopathologically characterized breast cancer tissues were eluted with phosphate buffered 0.9% sodium chloride solution, pH 7.2. The proteins were then characterized by polyacrylamide gel electrophoresis with and without prior treatment with sodium dodecyl sulfate. Approximately 65% of the breast cancer tissue eluates contained a prominent protein fraction with a molecular weight of 47,000 to 55,000 (p50). No such component was found in 15 of 17 eluates of benign breast tissue. Charge density studies disclosed that the p50 component included three populations of proteins that could be characterized according to the migration relative to gp55 derived from RIII murine mammary tumor virus, namely, fast (F p50), intermediate (I p50), and slow (S p50). Prognostically favorable pathologic characteristics, i.e., stage, nuclear grade, and lymphoreticuloendothelial responses, were proportionately most frequently found among S p50 breast cancers and were least frequently found among F p50 breast cancers. It appears that the S p50 component acts in vivo as a prognostically significant immunogen. Further knowledge of the relation between protein characteristics and clinicopathologic features of human breast cancers would contribute to the understanding of mammary carcinogenesis and biological behavior.
|Original language||English (US)|
|State||Published - Dec 1 1976|
All Science Journal Classification (ASJC) codes
- Cancer Research