Production of ethanol using enhanced ethanol-resistant mutants of E. coli Fbr5

Brian G. Lefebvre, Mariano J. Savelski, Gregory B. Hecht

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Ethanol derived from bacterial fermentation is a potential alternative fuel source. Ethanol resistant FBR5 mutants were isolated by subjecting independent cultures to multiple passages of alcohol challenges. Mutants were isolated from normal aerobic spread plates and from the anaerobic regions of pour plates. Pilot fermentations were conducted under anaerobic conditions at 30°C in LB media supplemented with 120 g/L xylose. One aerobic mutant strain produced ∼ 20% more ethanol than the parent FBR5 strain. The highest ethanol yields were obtained with an anaerobic mutant strain, which produced 60% more ethanol than the parent. Fermentation results under optimized conditions and ethanol yields of additional mutants were discussed. This is an abstract of a paper presented at the AIChE Annual Meeting and Fall Showcase (Cincinnati, OH 10/30/2005-11/4/2005).

Original languageEnglish (US)
Title of host publication05AIChE
Subtitle of host publication2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings
PublisherAmerican Institute of Chemical Engineers
ISBN (Print)0816909962, 9780816909964
StatePublished - Jan 1 2005
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Publication series

NameAIChE Annual Meeting Conference Proceedings
Volume2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

All Science Journal Classification (ASJC) codes

  • Energy(all)

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  • Cite this

    Lefebvre, B. G., Savelski, M. J., & Hecht, G. B. (2005). Production of ethanol using enhanced ethanol-resistant mutants of E. coli Fbr5. In 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Conference Proceedings (AIChE Annual Meeting Conference Proceedings; Vol. 2005). American Institute of Chemical Engineers.