Preliminary crystallographic characterization of ricin agglutinin

E. C. Sweeney, A. G. Tonevitsky, D. E. Temiakov, I. I. Agapov, S. Saward, R. A. Palmer

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


The quaternary structure of ricin agglutinin (RCA) has been determined by x-ray crystallography. The refined structure of ricin proved to be a successful search model using the molecular replacement method of phase determination. RCA forms an elongated molecule of dimensions 120 Å x 60 Å x 40 Å with two A chains at the center and a B chain at each end. The A chains are covalently associated via a disulfide bridge between Cys 156 of both chains. Additional contacts at residues 114-115 stabilize the dimer interface. The covalent association of RCAA chains was confirmed by gel filtration under reducing and nonreducing conditions.

Original languageEnglish (US)
Pages (from-to)586-589
Number of pages4
JournalProteins: Structure, Function and Genetics
Issue number4
StatePublished - 1997

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology


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