The quaternary structure of ricin agglutinin (RCA) has been determined by x-ray crystallography. The refined structure of ricin proved to be a successful search model using the molecular replacement method of phase determination. RCA forms an elongated molecule of dimensions 120 Å x 60 Å x 40 Å with two A chains at the center and a B chain at each end. The A chains are covalently associated via a disulfide bridge between Cys 156 of both chains. Additional contacts at residues 114-115 stabilize the dimer interface. The covalent association of RCAA chains was confirmed by gel filtration under reducing and nonreducing conditions.
|Original language||English (US)|
|Number of pages||4|
|Journal||Proteins: Structure, Function and Genetics|
|State||Published - 1997|
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology