Precise spatial positioning of chromosomes during prometaphase: Evidence for chromosomal order

Robert Nagele, Theresa Freeman, Lydia McMorrow, Hsin Yi Lee

Research output: Contribution to journalArticlepeer-review

136 Scopus citations

Abstract

The relative locations of several chromosomes within wheel-shaped prometaphase chromosome rosettes of human fibroblasts and HeLa cells were determined with fluorescence hybridization. Homologs were consistently positioned on opposite sides of the rosette, which suggests that chromosomes are separated into two haploid sets, each derived from one parent. The relative locations of chromosomes on the rosette were mapped by dual hybridizations. The data suggest that the chromosome orders within the two haploid sets are antiparallel. This chromosome arrangement in human cells appears to be both independent of cell type- and species-specific and may influence chromosome topology throughout the cell cycle.

Original languageEnglish (US)
Pages (from-to)1831
Number of pages1
JournalScience
Volume270
Issue number5243
DOIs
StatePublished - 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Precise spatial positioning of chromosomes during prometaphase: Evidence for chromosomal order'. Together they form a unique fingerprint.

Cite this