TY - JOUR
T1 - Oxidant damage to DNA and pregnancy outcome
AU - Scholl, T. O.
AU - Stein, T. P.
N1 - Funding Information:
The authors wish to thank the staff of Osborn Family Health Center, Our Lady of Lourdes Hospital and St John the Baptist Prenatal Clinic for access to patients. This work was supported in part by grant HD38329 from the National Institute of Child Health and Human Development.
PY - 2001
Y1 - 2001
N2 - Objective: DNA is susceptible to oxidation and is constantly being damaged and repaired in living cells. The most abundant of the nucleoside oxidation products is 8-oxo-7,8 dihydro-2 deoxyguanosine (8 OH-dG). Our objective was to determine whether oxidative damage to DNA, as measured by 8 OH-dG, is increased with poor pregnancy outcome. Method: We utilized a case-control design to study oxidative damage to DNA during an ongoing prospective study. Cases (n = 18) included all women giving birth to a low-birth-weight (< 2500 g) or growth-restricted (< 10th centile) or preterm infant (< 37 completed weeks). Controls (n = 34) were selected at random from women with normal pregnancies. Urine samples were obtained early in the third trimester (28 ± 2 weeks) and normalized to creatinine. Diet was assessed at three points during pregnancy. Results: Cases had significant (p < 0.05) increases in maternal urinary 8 OH-dG excretion at week 28, when all cases were considered and when cases were defined as those who delivered a low-birth-weight infant. 8 OH-dG excretion, in turn, correlated positively with saturated fat in the maternal diet. Conclusion: This study suggests that gravidas with poor pregnancy outcome have increased oxidative damage to their DNA early in the third trimester of pregnancy.
AB - Objective: DNA is susceptible to oxidation and is constantly being damaged and repaired in living cells. The most abundant of the nucleoside oxidation products is 8-oxo-7,8 dihydro-2 deoxyguanosine (8 OH-dG). Our objective was to determine whether oxidative damage to DNA, as measured by 8 OH-dG, is increased with poor pregnancy outcome. Method: We utilized a case-control design to study oxidative damage to DNA during an ongoing prospective study. Cases (n = 18) included all women giving birth to a low-birth-weight (< 2500 g) or growth-restricted (< 10th centile) or preterm infant (< 37 completed weeks). Controls (n = 34) were selected at random from women with normal pregnancies. Urine samples were obtained early in the third trimester (28 ± 2 weeks) and normalized to creatinine. Diet was assessed at three points during pregnancy. Results: Cases had significant (p < 0.05) increases in maternal urinary 8 OH-dG excretion at week 28, when all cases were considered and when cases were defined as those who delivered a low-birth-weight infant. 8 OH-dG excretion, in turn, correlated positively with saturated fat in the maternal diet. Conclusion: This study suggests that gravidas with poor pregnancy outcome have increased oxidative damage to their DNA early in the third trimester of pregnancy.
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U2 - 10.1080/jmf.10.3.182.185-10
DO - 10.1080/jmf.10.3.182.185-10
M3 - Article
C2 - 11444787
AN - SCOPUS:0034913027
SN - 1057-0802
VL - 10
SP - 182
EP - 185
JO - Journal of Maternal-Fetal Medicine
JF - Journal of Maternal-Fetal Medicine
IS - 3
ER -