TY - GEN
T1 - Optimization of macromer density in human MSC-laden Hyaluronic Acid (HA) hydrogels
AU - Kim, M.
AU - Garrity, S.
AU - Erickson, I. E.
AU - Huang, A. H.
AU - Burdick, J. A.
AU - Mauck, R. L.
PY - 2012
Y1 - 2012
N2 - Mesenchymal stem cells are attractive cell type and can undergo chondrogenesis in various 3D platforms. Hyaluronic acid (HA) hydrogel, a natural constituent of the cartilage extracellular matrix, provides a biologically relevant interface for encapsulated cells. While MSC-laden HA constructs can produce native mechanical properties using cells from animal sources, clinical repair will depend on successful translation of these findings to human MSCs (hMSCs). To optimize chondrogenesis, we assessed the ability of hMSCs to undergo chondrogenesis in varying macromer concentration HA gels. Variation in this parameter influenced construct mechanical and biochemical properties. In 1% methacrylated HA (MeHA), equilibrium modulus and GAG content were higher (86kPa (E Y) and 2.16%) than in 2% MeHA constructs (50 kPa, 1.62% GAG). However, greater contractility occurred in 1% MeHA (-36.25%/-24.25%; Thickness/diameter) compared to 2% MeHA (-20.57%/1.02%) constructs. This study provides new insight into optimized macromer densities for hMSC-based cartilage tissue engineering using HA hydrogels.
AB - Mesenchymal stem cells are attractive cell type and can undergo chondrogenesis in various 3D platforms. Hyaluronic acid (HA) hydrogel, a natural constituent of the cartilage extracellular matrix, provides a biologically relevant interface for encapsulated cells. While MSC-laden HA constructs can produce native mechanical properties using cells from animal sources, clinical repair will depend on successful translation of these findings to human MSCs (hMSCs). To optimize chondrogenesis, we assessed the ability of hMSCs to undergo chondrogenesis in varying macromer concentration HA gels. Variation in this parameter influenced construct mechanical and biochemical properties. In 1% methacrylated HA (MeHA), equilibrium modulus and GAG content were higher (86kPa (E Y) and 2.16%) than in 2% MeHA constructs (50 kPa, 1.62% GAG). However, greater contractility occurred in 1% MeHA (-36.25%/-24.25%; Thickness/diameter) compared to 2% MeHA (-20.57%/1.02%) constructs. This study provides new insight into optimized macromer densities for hMSC-based cartilage tissue engineering using HA hydrogels.
UR - http://www.scopus.com/inward/record.url?scp=84862741762&partnerID=8YFLogxK
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U2 - 10.1109/NEBC.2012.6207038
DO - 10.1109/NEBC.2012.6207038
M3 - Conference contribution
AN - SCOPUS:84862741762
SN - 9781467311410
T3 - 2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012
SP - 211
EP - 212
BT - 2012 38th Annual Northeast Bioengineering Conference, NEBEC 2012
T2 - 38th Annual Northeast Bioengineering Conference, NEBEC 2012
Y2 - 16 March 2012 through 18 March 2012
ER -