Abstract
Photochemical characteristics and substrate properties of four newly synthesized dCTP analogues: N4-[2-(2-nitro-5-azidobenzoylamino)ethyl]-, N4-[2-(4-azidotetrafluorobenzylideneaminooxymethylcarbamoyl)ethyl]-, N4-[4-(4-azidotetrafluorobenzylideneaminooxy)butyloxy]-, and N4-[4-(4-azidotetrafluorobenzylidene hydrazinocarbonyl)butylcarbamoyl]-, and N 4-[4-(4-azidotetrafluorobenzylideneaminooxy)butyloxy]-2′- deoxycytidine 5′-triphosphates as well as those of the earlier described N4-[2-(4-azidotetrafluorobenzoylamino)ethyl]- and 5-[E-3-(4-azidotetrafluorobenzoylamino)-1-propenyl)]-2′-deoxycytidine 5′-triphosphates were compared. When being irradiated with UV light at a wavelength of 303-313 nm, the new analogues demonstrated greater than 10-fold higher photoactivity as compared with the old compounds. The first three new compounds were utilized by HIV-1 reverse transcriptase as dCTP and dTTP, while the last derivative was recognized only as dTTP. Once incorporated into the primer 3′-terminus, none of the analogues synthesized terminated further primer elongation with natural triphosphates.
Original language | English (US) |
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Pages (from-to) | 584-585 |
Number of pages | 2 |
Journal | Bioorganicheskaya Khimiya |
Volume | 23 |
Issue number | 7 |
State | Published - Nov 1997 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- General Medicine