Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Lloyd D. Fricker; Alexandre K. Tashima; Amanda K. Fakira; Ute Hochgeschwender; William C. Wetsel; Lakshmi A. Devi

doi:10.1016/j.chembiol.2020.11.003

Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Lloyd D. Fricker
, Alexandre K. Tashima
, Amanda K. Fakira
, Ute Hochgeschwender
, William C. Wetsel
, Lakshmi A. Devi

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.

Original language	English (US)
Pages (from-to)	105-112.e4
Journal	Cell Chemical Biology
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/j.chembiol.2020.11.003
State	Published - Jan 21 2021
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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10.1016/j.chembiol.2020.11.003

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abstract = "We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.",

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AU - Fakira, Amanda K.

AU - Hochgeschwender, Ute

AU - Wetsel, William C.

AU - Devi, Lakshmi A.

PY - 2021/1/21

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AB - We report a method to identify neuropeptides in specific cell types, using mice with a conditional deletion of carboxypeptidase E. It was validated by deleting CPE in proopiomelanocortin cells. Surprisingly, these mice have greatly reduced levels of the anorexigenic peptide α-MSH but are not obese.

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Neuropeptidomic Analysis of a Genetically Defined Cell Type in Mouse Brain and Pituitary

Abstract

All Science Journal Classification (ASJC) codes

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