TY - JOUR
T1 - Mutations in the rod outer segment membrane guanylate cyclase in a cone- rod dystrophy cause defects in calcium signaling
AU - Duda, Teresa
AU - Krishnan, Anuradha
AU - Venkataraman, Venkateswar
AU - Lange, Christian
AU - Koch, Karl Wilhelm
AU - Sharma, Rameshwar K.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1999/10/19
Y1 - 1999/10/19
N2 - Rod outer segment guanylate cyclase 1 (ROS-GC1) is a member of the subfamily of Ca2+-regulated membrane guanylate cyclases; and it is pivotal for vertebrate phototransduction. Two opposing regulatory modes control the activity of ROS-GC1. At nanomolar concentrations of Ca2+, ROS-GC1 is activated by Ca2+-binding proteins named guanylate cyclase activating proteins (GCAPs). However, at micromolar concentrations of Ca2+, ROS-GC1 is stimulated by S100β [also named calcium-dependent (CD) GCAP]. This mode is not linked with phototransduction; instead, it is predicted to be involved in retinal synaptic activity. Two point mutations, E786D and R787C, in ROS-GC1 have been connected with cone-rod dystrophy (CORD6), with only one type of point mutation occurring in each family. The present study shows that the E786D mutation has no effect on the basal catalytic activity of ROS-GC1 and on its activation by GCAP1 and S100β; however, the mutated cyclase becomes more activated by GCAP2. The R787C mutation has three consequences: (1) it causes major damage to the basal cyclase activity, (2) it makes the cyclase 5-fold more sensitive to activation by GCAP1; and 3) converts the cyclase into a form that is less sensitive to activation by GCAP2 and S100β. Thus, the two CORD6-linked mutations in ROS-GC1, which occur at adjacent positions, result in vastly different biochemical phenotypes, and they are connected with very specific molecular defects in the Ca2+ switching components of the cyclase. These defects, in turn, are proposed to have a profound effect on both the machinery of phototransduction and the retinal synapse. The study for the first time defines the biochemistry of CORD6 pathology in precise molecular terms.
AB - Rod outer segment guanylate cyclase 1 (ROS-GC1) is a member of the subfamily of Ca2+-regulated membrane guanylate cyclases; and it is pivotal for vertebrate phototransduction. Two opposing regulatory modes control the activity of ROS-GC1. At nanomolar concentrations of Ca2+, ROS-GC1 is activated by Ca2+-binding proteins named guanylate cyclase activating proteins (GCAPs). However, at micromolar concentrations of Ca2+, ROS-GC1 is stimulated by S100β [also named calcium-dependent (CD) GCAP]. This mode is not linked with phototransduction; instead, it is predicted to be involved in retinal synaptic activity. Two point mutations, E786D and R787C, in ROS-GC1 have been connected with cone-rod dystrophy (CORD6), with only one type of point mutation occurring in each family. The present study shows that the E786D mutation has no effect on the basal catalytic activity of ROS-GC1 and on its activation by GCAP1 and S100β; however, the mutated cyclase becomes more activated by GCAP2. The R787C mutation has three consequences: (1) it causes major damage to the basal cyclase activity, (2) it makes the cyclase 5-fold more sensitive to activation by GCAP1; and 3) converts the cyclase into a form that is less sensitive to activation by GCAP2 and S100β. Thus, the two CORD6-linked mutations in ROS-GC1, which occur at adjacent positions, result in vastly different biochemical phenotypes, and they are connected with very specific molecular defects in the Ca2+ switching components of the cyclase. These defects, in turn, are proposed to have a profound effect on both the machinery of phototransduction and the retinal synapse. The study for the first time defines the biochemistry of CORD6 pathology in precise molecular terms.
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U2 - 10.1021/bi9915972
DO - 10.1021/bi9915972
M3 - Article
C2 - 10529237
AN - SCOPUS:0344069786
VL - 38
SP - 13912
EP - 13919
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 42
ER -