TY - JOUR
T1 - Multisubunit RNA polymerases melt only a single DNA base pair downstream of the active site
AU - Kashkina, Ekaterina
AU - Anikin, Michael
AU - Brueckner, Florian
AU - Lehmann, Elisabeth
AU - Kochetkov, Sergey N.
AU - McAllister, William T.
AU - Cramer, Patrick
AU - Temiakov, Dmitry
PY - 2007/7/27
Y1 - 2007/7/27
N2 - To extend the nascent transcript, RNA polymerases must melt the DNA duplex downstream from the active site to expose the next acceptor base for substrate binding and incorporation. A number of mechanisms have been proposed to account for the manner in which the correct substrate is selected, and these differ in their predictions as to how far the downstream DNA is melted. Using fluorescence quenching experiments, we provide evidence that cellular RNA polymerases from bacteria and yeast melt only one DNA base pair downstream from the active site. These data argue against a model in which multiple NTPs are lined up downstream of the active site.
AB - To extend the nascent transcript, RNA polymerases must melt the DNA duplex downstream from the active site to expose the next acceptor base for substrate binding and incorporation. A number of mechanisms have been proposed to account for the manner in which the correct substrate is selected, and these differ in their predictions as to how far the downstream DNA is melted. Using fluorescence quenching experiments, we provide evidence that cellular RNA polymerases from bacteria and yeast melt only one DNA base pair downstream from the active site. These data argue against a model in which multiple NTPs are lined up downstream of the active site.
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U2 - 10.1074/jbc.C700098200
DO - 10.1074/jbc.C700098200
M3 - Article
C2 - 17526498
AN - SCOPUS:34547555850
SN - 0021-9258
VL - 282
SP - 21578
EP - 21582
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 30
ER -