Multiple functions of yeast mitochondrial transcription factor Mtf1p during initiation

Maria Savkina, Dmitry Temiakov, William T. McAllister, Michael Anikin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Transcription of the yeast mitochondrial genome is carried out by an RNA polymerase (Rpo41p) that is related to single subunit bacteriophage RNA polymerases but requires an additional factor (Mtf1p) for initiation. In this work we show that Mtf1p is involved in multiple roles during initiation including discrimination of upstream base pairs in the promoter, initial melting of three to four base pairs around the site of transcript initiation, and suppression of nonspecific initiation. It, thus, appears that Mtf1p is functionally analogous to initiation factors of multisubunit RNA polymerases, such as σ. Photocross-linking experiments reveal close proximity between Mtf1p and the promoter DNA and show that the C-terminal domain makes contacts with the template strand in the vicinity of the start site. Interestingly, Mtf1p is related to a class of RNA methyltransferases, suggesting an early evolutionary link between RNA synthesis and processing.

Original languageEnglish (US)
Pages (from-to)3957-3964
Number of pages8
JournalJournal of Biological Chemistry
Volume285
Issue number6
DOIs
StatePublished - Feb 5 2010

Fingerprint

DNA-Directed RNA Polymerases
Yeast
Peptide Initiation Factors
Transcription Factors
Yeasts
Base Pairing
RNA
Bacteriophages
Mitochondrial Genome
Methyltransferases
Transcription
Freezing
Melting
Genes
DNA
Processing
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Savkina, Maria ; Temiakov, Dmitry ; McAllister, William T. ; Anikin, Michael. / Multiple functions of yeast mitochondrial transcription factor Mtf1p during initiation. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 6. pp. 3957-3964.
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Multiple functions of yeast mitochondrial transcription factor Mtf1p during initiation. / Savkina, Maria; Temiakov, Dmitry; McAllister, William T.; Anikin, Michael.

In: Journal of Biological Chemistry, Vol. 285, No. 6, 05.02.2010, p. 3957-3964.

Research output: Contribution to journalArticle

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