MicroRNAs downregulated in neuropathic pain regulate MeCP2 and BDNF related to pain sensitivity

Melissa T. Manners; Yuzhen Tian; Zhaolan Zhou; Seena K. Ajit

doi:10.1016/j.fob.2015.08.010

MicroRNAs downregulated in neuropathic pain regulate MeCP2 and BDNF related to pain sensitivity

Melissa T. Manners, Yuzhen Tian, Zhaolan Zhou, Seena K. Ajit

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Nerve injury induces chronic pain and dysregulation of microRNAs in dorsal root ganglia (DRG). Several downregulated microRNAs are predicted to target Mecp2. MECP2 mutations cause Rett syndrome and these patients report decreased pain perception. We confirmed MeCP2 upregulation in DRG following nerve injury and repression of MeCP2 by miRNAs in vitro. MeCP2 regulates brain-derived neurotrophic factor (BDNF) and downregulation of MeCP2 by microRNAs decreased Bdnf in vitro. MeCP2 T158A mice exhibited reduced mechanical sensitivity and Mecp2-null and MeCP2 T158A mice have decreased Bdnf in DRG. MeCP2-mediated regulation of Bdnf in the DRG could contribute to altered pain sensitivity.

Original language	English (US)
Pages (from-to)	733-740
Number of pages	8
Journal	FEBS Open Bio
Volume	5
DOIs	https://doi.org/10.1016/j.fob.2015.08.010
State	Published - 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.fob.2015.08.010

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title = "MicroRNAs downregulated in neuropathic pain regulate MeCP2 and BDNF related to pain sensitivity",

abstract = "Nerve injury induces chronic pain and dysregulation of microRNAs in dorsal root ganglia (DRG). Several downregulated microRNAs are predicted to target Mecp2. MECP2 mutations cause Rett syndrome and these patients report decreased pain perception. We confirmed MeCP2 upregulation in DRG following nerve injury and repression of MeCP2 by miRNAs in vitro. MeCP2 regulates brain-derived neurotrophic factor (BDNF) and downregulation of MeCP2 by microRNAs decreased Bdnf in vitro. MeCP2 T158A mice exhibited reduced mechanical sensitivity and Mecp2-null and MeCP2 T158A mice have decreased Bdnf in DRG. MeCP2-mediated regulation of Bdnf in the DRG could contribute to altered pain sensitivity.",

author = "Manners, {Melissa T.} and Yuzhen Tian and Zhaolan Zhou and Ajit, {Seena K.}",

note = "Publisher Copyright: {\textcopyright} 2015 The Authors.",

year = "2015",

doi = "10.1016/j.fob.2015.08.010",

language = "English (US)",

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journal = "FEBS Open Bio",

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T1 - MicroRNAs downregulated in neuropathic pain regulate MeCP2 and BDNF related to pain sensitivity

AU - Manners, Melissa T.

AU - Tian, Yuzhen

AU - Zhou, Zhaolan

AU - Ajit, Seena K.

PY - 2015

Y1 - 2015

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AB - Nerve injury induces chronic pain and dysregulation of microRNAs in dorsal root ganglia (DRG). Several downregulated microRNAs are predicted to target Mecp2. MECP2 mutations cause Rett syndrome and these patients report decreased pain perception. We confirmed MeCP2 upregulation in DRG following nerve injury and repression of MeCP2 by miRNAs in vitro. MeCP2 regulates brain-derived neurotrophic factor (BDNF) and downregulation of MeCP2 by microRNAs decreased Bdnf in vitro. MeCP2 T158A mice exhibited reduced mechanical sensitivity and Mecp2-null and MeCP2 T158A mice have decreased Bdnf in DRG. MeCP2-mediated regulation of Bdnf in the DRG could contribute to altered pain sensitivity.

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MicroRNAs downregulated in neuropathic pain regulate MeCP2 and BDNF related to pain sensitivity

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