Mechanisms Revealed Through General Anesthetic Photolabeling

Brian P. Weiser, Kellie A. Woll, William P. Dailey, Roderic G. Eckenhoff

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

General anesthetic photolabels are used to reveal molecular targets and molecular binding sites of anesthetic ligands. After identification, the relevance of anesthetic substrates or binding sites can be tested in biological systems. Halothane and photoactive analogs of isoflurane, propofol, etomidate, neurosteroids, anthracene, and long chain alcohols have been used in anesthetic photolabeling experiments. Interrogated protein targets include the nicotinic acetylcholine receptor, GABAA receptor, tubulin, leukocyte function-associated antigen-1, and protein kinase C. In this review, we summarize insights revealed by photolabeling these targets, as well as general features of anesthetics, such as their propensity to partition to mitochondria and bind voltage-dependent anion channels. The theory of anesthetic photolabel design and the experimental application of photoactive ligands are also discussed.

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalCurrent Anesthesiology Reports
Volume4
Issue number1
DOIs
StatePublished - Mar 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

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