Mapping of a contact for the RNA 3' terminus in the largest subunit of RNA polymerase

S. Borukhov, J. Lee, A. Goldfarb

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Stalled elongation complexes of Escherichia coli RNA polymerase were prepared carrying the photo-cross-linkable 8-azido derivative of adenine at the 3'-terminus of the nascent RNA chain. Ultraviolet irradiation of such complexes resulted in the cross-linking of radiolabeled RNA exclusively to the β' subunit of RNA polymerase. The adduct was mapped between Met932 and Trp1020 in the linear sequence of the β' polypeptide using specific chemical degradation of the cross-linked species.

Original languageEnglish (US)
Pages (from-to)23932-23935
Number of pages4
JournalJournal of Biological Chemistry
Volume266
Issue number35
StatePublished - Dec 1 1991

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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