Stalled elongation complexes of Escherichia coli RNA polymerase were prepared carrying the photo-cross-linkable 8-azido derivative of adenine at the 3'-terminus of the nascent RNA chain. Ultraviolet irradiation of such complexes resulted in the cross-linking of radiolabeled RNA exclusively to the β' subunit of RNA polymerase. The adduct was mapped between Met932 and Trp1020 in the linear sequence of the β' polypeptide using specific chemical degradation of the cross-linked species.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Biological Chemistry|
|State||Published - Dec 1 1991|
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology