Mam33 promotes cytochrome c oxidase subunit i translation in Saccharomyces cerevisiae mitochondria

Gabrielle A. Roloff, Michael F. Henry

    Research output: Contribution to journalArticlepeer-review

    13 Scopus citations

    Abstract

    Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from generating free oxygen radicals. In the yeast Saccharomyces cerevisiae, the nuclear-encoded proteins Mss51 and Pet309 specifically activate mitochondrial translation of the largest subunit, Cox1. Here we report that Mam33 is a third COX1 translational activator in yeast mitochondria. Mam33 is required for cells to adapt efficiently from fermentation to respiration. In the absence of Mam33, Cox1 translation is impaired, and cells poorly adapt to respiratory conditions because they lack basal fermentative levels of Cox1.

    Original languageEnglish (US)
    Pages (from-to)2885-2894
    Number of pages10
    JournalMolecular biology of the cell
    Volume26
    Issue number16
    DOIs
    StatePublished - Aug 15 2015

    All Science Journal Classification (ASJC) codes

    • Molecular Biology
    • Cell Biology

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