Mam33 promotes cytochrome c oxidase subunit i translation in Saccharomyces cerevisiae mitochondria

Gabrielle A. Roloff, Michael F. Henry

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from generating free oxygen radicals. In the yeast Saccharomyces cerevisiae, the nuclear-encoded proteins Mss51 and Pet309 specifically activate mitochondrial translation of the largest subunit, Cox1. Here we report that Mam33 is a third COX1 translational activator in yeast mitochondria. Mam33 is required for cells to adapt efficiently from fermentation to respiration. In the absence of Mam33, Cox1 translation is impaired, and cells poorly adapt to respiratory conditions because they lack basal fermentative levels of Cox1.

Original languageEnglish (US)
Pages (from-to)2885-2894
Number of pages10
JournalMolecular Biology of the Cell
Volume26
Issue number16
DOIs
StatePublished - Aug 15 2015

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Electron Transport Complex IV
Saccharomyces cerevisiae
Mitochondria
Yeasts
Electron Transport
Nuclear Proteins
Mitochondrial DNA
Fermentation
Free Radicals
Reactive Oxygen Species
Respiration
Proteins
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "Three mitochondrial DNA-encoded proteins, Cox1, Cox2, and Cox3, comprise the core of the cytochrome c oxidase complex. Gene-specific translational activators ensure that these respiratory chain subunits are synthesized at the correct location and in stoichiometric ratios to prevent unassembled protein products from generating free oxygen radicals. In the yeast Saccharomyces cerevisiae, the nuclear-encoded proteins Mss51 and Pet309 specifically activate mitochondrial translation of the largest subunit, Cox1. Here we report that Mam33 is a third COX1 translational activator in yeast mitochondria. Mam33 is required for cells to adapt efficiently from fermentation to respiration. In the absence of Mam33, Cox1 translation is impaired, and cells poorly adapt to respiratory conditions because they lack basal fermentative levels of Cox1.",
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Mam33 promotes cytochrome c oxidase subunit i translation in Saccharomyces cerevisiae mitochondria. / Roloff, Gabrielle A.; Henry, Michael F.

In: Molecular Biology of the Cell, Vol. 26, No. 16, 15.08.2015, p. 2885-2894.

Research output: Contribution to journalArticle

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